Adiposity in childhood brain tumors: a report from the canadian study of determinants of endometabolic health in children (candecide study)

ABSTRACT Children with brain tumors (CBT) are at high risk of cardiovascular diseases and type 2 diabetes compared to the general population. Recently, adiposity has been reported to be more

informative for cardiometabolic risk stratification than body mass index (BMI) in the general population. The goal of this study is to describe the adiposity phenotype in CBT, and to

establish adiposity determinants. We recruited CBT (n = 56) and non-cancer controls (n = 106). Percent body fat (%FM), waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR) were measured

to determine total and central adiposity, respectively. Regression analyses were used to evaluate adiposity determinants. CBT had higher total and central adiposity compared to non-cancer

controls despite having similar BMI measurements. Those with tumors at the supratentorial region had increased total and central adiposity, while those who received radiotherapy had

increased total adiposity. In conclusion, CBT have increased total and central adiposity in the presence of similar BMI levels when compared to non-cancer controls. Adiposity, especially

central adiposity, is a potential cardiometabolic risk factor present relatively early in life in CBT. Defining interventions to target adiposity may improve long-term outcomes by preventing

cardiometabolic disorders in CBT. SIMILAR CONTENT BEING VIEWED BY OTHERS THE TRIPONDERAL MASS INDEX AS A MEASURE OF ADIPOSITY IN PEDIATRIC SURVIVORS OF ACUTE LYMPHOBLASTIC LEUKEMIA: A

CROSS-SECTIONAL STUDY Article Open access 26 January 2022 HIGH MOLECULAR WEIGHT ADIPONECTIN LEVELS ARE INVERSELY ASSOCIATED WITH ADIPOSITY IN PEDIATRIC BRAIN TUMOR SURVIVORS Article Open

access 29 October 2020 LONGITUDINAL BODY MASS INDEX AND CANCER RISK: A COHORT STUDY OF 2.6 MILLION CATALAN ADULTS Article Open access 30 June 2023 INTRODUCTION Brain tumors are the most

common pediatric solid tumors1. Groundbreaking discoveries in tumor biology and advances in diagnosis and therapy have significantly improved the survival of many of these children2. As the

number of survivors has risen, it has become evident that this group is at risk of developing chronic morbidities3,4 and premature mortality5,6. Recent evidence suggests that adult survivors

of childhood brain tumors are at risk of cardiovascular diseases, including stroke, cardiac events, and type 2 diabetes7,8,9,10. As obesity is an independent risk factor for cardiometabolic

disorders in the general population, it may provide an explanation of the added cardiometabolic risk in survivors11. However, when obesity rates are measured by using Body Mass Index (BMI),

children with brain tumors (CBT) are reported to have BMI levels that are either close to or slightly higher than rates in the general population, which does not seem to explain this

increased cardiometabolic risk in survivors12,13. While BMI is the most widely used clinical measure of obesity, it does not distinguish the relative contribution of fat, muscle, or bone to

body mass, which are considerably variable in growing children14. On the other hand, adiposity may be a better measurement to determine cardiometabolic risk in CBT. Adiposity is defined as

the presence of fat in and outside the adipose tissue, including muscle and hepatic fat depots. The adipose depot is composed of a subcutaneous compartment, which is considered protective

against cardiometabolic risk15,16. On the other hand, the visceral adipose compartment secretes inflammatory cytokines which can lead to insulin resistance, and is linked to adverse

cardiometabolic outcomes17. Measures of total adiposity (fat mass percentage; %FM) and central adiposity, including waist-to-hip ratio (WHR) and waist-to-height ratio (WHtR), have been shown

to be more robust predictors of cardiometabolic health and risk compared to BMI18,19,20,21,22,23,24, with WHtR emerging as a strong indicator of intra-abdominal fat25. However, adiposity is

not routinely measured in children, including pediatric cancer patients. While brain tumors are a heterogeneous group, a common tumor classically reported to be associated with obesity is

craniopharyngioma26. There have been very few reports on the evaluation of obesity in other brain tumor subtypes and beyond hypothalamic obesity27,28. As BMI-based obesity rates are similar

between CBT and controls yet CBT have high risk of cardiometabolic disorders, we hypothesized that CBT, excluding craniopharyngioma, have higher adiposity when compared to non-cancer

controls. This excess adiposity may contribute to adverse cardiometabolic outcomes and premature mortality. A secondary aim of this study was to investigate the determinants of adiposity in

CBT. RESULTS We included 56 CBT (n = 23 female) and 106 non-cancer controls (n = 51 female) in this study. The characteristics of the study population are reported in Table 1. The two groups

were similar in terms of age (CBT: 5.20–42.70 years; controls: 5.40–18.80 years; p-value 0.59) and sex distribution (p-value 0.39). The CBT group had more participants in prepubertal stage

(n = 19, 33.90%) versus controls (n = 16, 15.10%). Age of diagnosis of brain tumor was 9.10 ± 4.90 years, and average time since diagnosis was 5.60 ± 5.10 years. As reported previously29,

CBT were shorter (150.60 ± 25.20 versus 161.70 ± 15.30 cm, p-value = 0.002) and weighed less (52.40 ± 24.10 versus 59.00 ± 20.80 kg, p-value = 0.02) than the control group. The %FM

correlated with central adiposity (Spearman’s rho test WHR 0.31, p-value < 0.001; WHtR 0.73, p-value < 0.001). Central adiposity measures were highly correlated with each other as well

(Spearman’s rho test 0.67, p-value < 0.001). The total screen time and sleep duration were similar between the two groups (Table 1). The most common tumor subtypes in participants

included gliomas (n = 34, 60.70%) and Primitive Neuroectodermal tumors (PNET)/medulloblastoma (n = 11, 19.60%) (Table 2). The tumors were distributed between supratentorial (n = 26, 46.40%)

and infratentorial regions (n = 30, 53.60%) (Table 2), with only 7 patients (12.50%) having tumors involving the hypothalamus. The therapeutic modalities were used in the management of brain

tumors are shown in Table 2. Surgery alone was the most common treatment modality (n = 18, 32.10%), followed by a combination of surgery, chemotherapy and radiotherapy (n = 15, 26.80%).

Chemotherapy alone was noted in five cases (8.90%), and radiotherapy alone was implemented in one patient (1.80%). Four patients (7.10%) received surgery and chemotherapy, and four (7.10%)

received surgery and radiotherapy; one received radiotherapy and chemotherapy (1.80%). In the 22 participants who received radiotherapy, the radiotherapy dosage was 47.10 ± 12.40 Gy. Sixteen

participants received craniospinal irradiation (72.70%), and six received cranial irradiation (27.30%). Eight patients were being managed with watch-and-wait strategy (14.30%). Post-therapy

endocrinopathies were observed in 14 (26.80%) CBT participants. Among this group, a single diagnosis was made in seven patients including hypothyroidism (n = 3, 21.40%), growth hormone

deficiency (n = 2, 14.30%), hypogonadism (n = 1, 7.10%), and precocious puberty (n = 1, 7.10%). The other seven patients had multiple hormonal deficiencies including hypothyroidism (n = 5,

35.70%), growth hormone deficiency (n = 6, 42.90%), hypogonadism (n = 4, 28.60%), adrenocorticotropic hormone deficiency (n = 4, 28.60%), diabetes insipidus (n = 3, 21.40%), and precocious

puberty (n = 1, 7.10%). All endocrinopathies were treated appropriately. ADIPOSITY PATTERNS IN CBT AND CONTROLS To determine if CBT have enhanced adiposity compared to non-cancer controls,

we used logistic regression analysis. CBT had higher total adiposity compared to controls (%FM 25.50 ± 9.60% versus 22.40 ± 9.30%; β = 1.51, 95% CI = 1.08, 2.10, p-value = 0.016). CBT also

had higher central adiposity compared to controls including higher WHR (0.87 ± 0.07 versus 0.82 ± 0.09; β = 7.53, 95% CI = 2.30, 24.64, p-value = 0.001) and a trend of higher WHtR (0.47 ± 

0.06 versus 0.45 ± 0.08; β = 0.34, 95% CI = 0.12, 1.02, p-value = 0.053). Importantly, there were no differences in BMI and overweight/obesity rates between CBT and non-cancer controls

(Table 1). BMI correlated with total adiposity (%FM) in CBT and controls (Spearman’s rho test CBT 0.50, p-value < 0.001; controls 0.76, p-value < 0.001). BMI also correlated with WHR

in controls but not in CBT (Spearman’s rho test CBT 0.41, p-value 0.12; controls 0.17, p-value 0.038). Furthermore, BMI correlated with WHtR in CBT and controls (Spearman’s rho test CBT

0.51, p-value < 0.001; controls 0.73, p-value < 0.001). These results demonstrate that CBT have higher total and central adiposity compared to non-cancer controls, in the presence of

similar obesity rates based on BMI measurements. DETERMINANTS OF ADIPOSITY IN SURVIVORS AND CONTROLS To define the determinants of adiposity, we conducted separate exploratory subgroup

analyses using multivariate linear regression for CBT and controls (Table 3 for CBT; Supplementary Table S1 for controls). Dietary data are included in Table 4. Females in the control group

had higher total adiposity, while males had increased WHR, and puberty was associated with all measures of adiposity. These trends were not noted in CBT. CBT with Supratentorial tumors had

increased total adiposity (β −1.83, SE 0.80, p-value 0.028), with trended association with central adiposity (WHR β −0.37, SE 0.21, p-value 0.08; WHtR β −0.53, SE 0.27, p-value 0.06) (Table

3). CBT who received radiotherapy had higher %FM (β = 1.65, SE 0.79, p-value = 0.046). However, radiotherapy type (craniospinal versus cranial irradiation) and radiation dose did not

correlate with %FM (Spearman’s rho test radiotherapy type r 0.13, p-value 0.57; Dose r 0.24, p-value 0.36), WHR (Spearman’s rho test radiotherapy type r 0.18, p-value 0.43; Dose r 0.33,

p-value 0.17), or WHtR (Spearman’s rho test radiotherapy type r 0.10, p-value 0.67; Dose r 0.24, p-value 0.3)’. While 27 (48.2%) CBT were treated with corticosteroids, there was no

association between steroid use and %FM (β = 0.68, SE 0.62, p-value = 0.28), WHR (β = 0.04, SE 0.19, p-value = 0.81), or WHtR (β = 0.21, SE 0.21, p-value = 0.32) (Table 3). When examining

the contribution of lifestyle factors (diet, physical activity, screen time, sleep duration) to adiposity in controls, physical inactivity trended with WHR, while screen time was associated

with WHtR. Diet and sleep duration were not associated with adiposity measures. None of the lifestyle factors were associated with total or central adiposity measures in CBT (Table 3 for

CBT; Supplementary Table S1 for controls; Diet data Table 4). DISCUSSION The improved survival rates of children with brain tumors have been hindered by premature mortality and the

development of morbidities. Of particular importance, recent evidence confirms that survivors are at risk of type 2 diabetes and cardiovascular diseases7,8,9,10. In this study, we

demonstrate that adiposity, one of the most important determinants of cardiometabolic risk, is enhanced in CBT when compared to non-cancer controls. Importantly, the adipose phenotype noted

in CBT is evident with equivalent overweight/obesity rates to controls based on BMI measurements. It has been reported that BMI can underestimate the prevalence of obesity in childhood

cancer survivors, including survivors of brain tumors18. Until further knowledge is generated of the potential role of early excess adiposity in programming future cardiometabolic risk in

CBT, there is a need to measure both BMI and adipose depots, and to continue to attempt to define their determinants. Our data are consistent with studies that used dual X-ray absorptiometry

(DXA) scans30, and reported the presence of higher total adiposity in cancer survivors who were treated with cranial irradiation30. The first study identified impaired mobility as an

association of adiposity; the second study recruited patients with different cancers including brain tumors, and used siblings as a control group. The latter study identified male sex and

screen time as risk factors of adiposity30. Our study population included CBT exclusively, with non-cancer controls as a comparison group. This may explain why the previously identified risk

factors were not associated with adiposity in our study. An important contribution of our study is that it provides evidence for the use of clinically feasible measures to determine

adiposity in CBT. This has important implications for settings where access to DXA is not practical or possible, allowing clinicians to estimate the adiposity patterns in their survivor

populations. Our data also demonstrate that tumor location and radiotherapy have important associations with adiposity. Supratentorial tumors were associated with enhanced total and central

adiposity, while radiotherapy was associated with excess total adiposity. While tumors and their treatment can lead to anatomical or functional hypothalamic-pituitary damage with pituitary

hormonal deficiencies31, disruption of hypothalamic satiety signaling and reduced basal metabolic rate that can drive obesity26, these factors may also contribute to excess adiposity. Our

results did not corroborate previous evidence of the association of higher doses of radiotherapy with obesity in childhood cancers, including brain tumors32,33. While these studies used BMI

to measure obesity, our results suggest that adiposity may be associated with radiotherapy regardless of dosage. Clarifying the effect of radiotherapy type, dosing and fractionation on

adiposity is an important question to address in CBT. Endocrinopathies have been reported to increase the risk of higher BMI, but their effect on adiposity patterns in CBT early on requires

further study, as these effects may become more apparent as CBT age. Given that radiation dosage is associated with hormonal abnormalities in cancer survivors, the effect of radiation dosage

on adiposity may have been masked in our population who were treated for existing endocrinopathies34. It has been reported that certain tumors including craniopharyngiomas, pilocytic

astrocytomas, and medulloblastomas are associated with elevated BMI33. We purposefully excluded craniopharyngiomas, to determine the contribution of other tumors to the adipose phenotype in

CBT. A larger sample size is needed to clarify whether adiposity is driven by specific tumor types. Several lifestyle factors are associated with obesity in the general pediatric population,

Including excess caloric intake from sugar-sweetened beverages, prolonged screen time, and short sleep duration. Physical inactivity has been a controversial determinant of obesity in

children35,36,37,38,39. While biological (sex), hormonal (puberty) and lifestyle factors were associated with adiposity in controls, none emerged as an explanation of the enhanced adiposity

profile in CBT, which was associated with tumor location and radiotherapy. The lack of association of diet with adiposity in our study is consistent with a study in craniopharyngioma

patients, which revealed that physical inactivity, and not nutritional factors, were associated with higher adiposity40. As our study is cross-sectional, one caveat is that the dietary

patterns may have changed from the time of diagnosis onwards. Longitudinal studies are needed to clarify the link between diet and adiposity in CBT. The association of physical inactivity

with childhood obesity and its use as a treatment for obesity has yielded inconsistent results41,42,43,44. In CBT, physical inactivity can be driven by treatment-related pulmonary and

cardiac dysfunction45,46, reduced muscle strength and fitness47, fatigue, sleep disturbance48, mental health issues, visual impairment, imbalance and pain49,50. Further studies on the

association of physical inactivity with adiposity, and fat mass modification by targeted interventions in CBT are needed. Our data suggest that within few years from having a brain tumor,

CBT are following the secular lifestyle trends noted in the general population. However, the effect of adopting these trends on adiposity and cardiometabolic risk in CBT can be

disproportionate, due to the added burden of the tumor and its treatment. Multipronged, personalized, and sustained interventions are needed in CBT, as adiposity is only one of many risk

factors that may respond to lifestyle alteration. There are several limitations to our study. While the WHR and WHtR demonstrated the presence of excess central adiposity in CBT, it is not

clear if this is due to subcutaneous or visceral fat depot expansion. It is also unclear yet if these adiposity patterns will be sustained as CBT age. In addition, due to the cost and

logistics involved we did not measure other fat depots including hepatic and intermyocellular fat. Larger sample size and longitudinal studies of the fat depots are needed starting at

diagnosis, to elucidate the evolution of the adiposity patterns in CBT. As the questionnaires were self-administered, the presence of recall bias is possible. However, this is less likely,

as the data collected were related to recent lifestyle factors, and the clinical data related to the tumor and its treatment were collected from the medical records. CONCLUSIONS In summary,

our study reveals that excess total and central adiposity are present in non-craniopharyngioma population of CBT compared to controls. Adiposity, especially central adiposity, is an

important cardiometabolic risk marker that appears in CBT within few years of their diagnosis. Tumor location and radiotherapy are important determinants of the noted adipose phenotype in

these patients. There is a need to understand the determinants of adiposity so that new therapies and prevention strategies can be developed to mitigate premature cardiovascular diseases and

type 2 diabetes and improve outcomes in CBT. METHODS PARTICIPANTS The participants in this study were recruited into the Canadian Study of Determinants of Endometabolic Health in Children

(CanDECIDE Study). This is a cohort study based at McMaster Children’s Hospital, a tertiary pediatric academic center in Hamilton, Ontario, Canada. The study protocol and feasibility have

been published51,52. The data reported are cross-sectional data collected at recruitment into the study. We consecutively recruited CBT from the neurooncology clinics, and non-cancer

controls were recruited from orthopedic clinics at the hospital and from the community. The orthopedic clinic controls included healthy children who suffered fractures or sprains and were

seen for evaluation. Importantly, all study measures were performed after the fractures or sprains have healed, and participants had returned to their usual lifestyle before the injury. The

recruitment period lasted from November 2012-March 2016. We recruited boys and girls, 5 years and older, who were free of infection for 15 days prior to participation in the study, with no

history of autoimmune diseases and not receiving immunosuppressive therapy for 15 days prior to inclusion. The exclusion criteria included active infection, autoimmune diseases, pregnancy or

inability to provide informed consent. CONSENT The Hamilton Integrated Research Ethics Board approved this study. Consent forms were signed by parents if the participants were less than 16

years old, or by the participants if they were 16 years or older53. Children 7–15 years of age also signed an additional assent form. Informed consent was obtained from all participants. The

study was conducted in accordance with appropriate clinical practice guidelines and national legal requirements. SOCIODEMOGRAPHIC AND CLINICAL DATA Data collected during the initial

encounter with potential participants included self-reported age and sex, and this was confirmed from the medical records. Additional data collected from the medical records included age at

diagnosis, tumor type, location, details of treatments received, and associated endocrinopathies and their treatment. Pubertal staging was assessed by pictorial Tanner pubertal staging in

girls (>8 year old) and boys (>9 year old)54. Height and weight were measured to the nearest one tenth of a centimeter and one tenth of a kilogram using a stadiometer and an electronic

weighing scale (Seca, USA), respectively. Body mass index (BMI) was calculated as kg/m2. BMI percentile was obtained using the Children’s BMI Tool for Schools55 and BMI z-score were

determined from the Centers for Disease Control and Prevention (CDC) growth chart56. Sitting systolic and diastolic blood pressures were measured twice using the right arm with an automated

blood pressure monitor (Welch Allyn, Inc., USA) and the average values of these two measurements are reported. The two commonly used methods to measure body fat include Dual-energy X-ray

absorptiometry (DXA) scan and bioelectrical impedance analysis (BIA)57. The latter is less expensive, easier to access and perform than DXA. In this study, we used BIA to measure %FM to

determine total adiposity. This method has been validated against DXA scans, and the two measures are highly correlated57. While the Tanita body fat monitor (Tanita Corporation, Illinois,

USA) is portable, it cannot be used on those 18 years and older. In this case, the InBody520 body composition analyzer (Biospace Co., Ltd, Korea) was used to measure %FM. High correlations

were established between the Tanita body fat monitor and the InBody520 body composition analyzer when tested on 5–17 year old children (r = 0.87; p-value = 0.001). Waist and hip

circumferences were measured to the nearest one tenth of a centimeter, using a spring-loaded measuring tape (OHAUS Corporation, Canada)58. Central adiposity was determined by calculating the

WHR and WHtR21. DIET Dietary intake was assessed as we previously reported52. Briefly, we used items from the Youth and Adolescent Food Frequency Questionnaire52,59,60. This is a

questionnaire developed in a US pediatric cohort, and includes questions about food intake based on average portion sizes of different dietary constituents. The number of servings per day

was calculated from the questionnaire by multiplying the frequency of consumption by portion size. Principal component analysis was used to analyze the dietary patterns in participants. This

analysis revealed four dietary patterns including prudent, western, high-protein and refined carbohydrate diets (Table 4). The prudent diet included high intake of fruits and vegetables.

The western diet included high intake of fried foods, desserts, baked goods, and refined foods (e.g., chips, snacks, candies). The high-protein diet included high intake of meat and eggs.

The refined carbohydrate diet included high intake of white bread and low intake of dark (whole grain) bread. PHYSICAL ACTIVITY Physical activity was measured using the Habitual Activity

Estimation Scale (HAES)61. The participants were asked to indicate their overall physical activity level as very inactive, inactive, somewhat inactive, somewhat active, active, or very

active. This data were used to report physical activity levels. The levels were dichotomized into active and inactive for statistical analyses. SLEEP Sleep duration (hours/day) was

calculated from the difference between the self-reported time the participant went to bed and woke up the next morning. Sleep duration calculated with this method has been shown to correlate

well with objective sleep quantification methods62. SCREEN TIME Total screen time (hours/day) was calculated from the sum of self-reported time spent watching television, using cell phone,

computer, computer games, and tablets. STATISTICAL ANALYSIS All analyses were performed with SPSS version 20 software63. Kolmogorov-Smirnov test was used to test for normality, and variables

with non-normal distribution were log-transformed. Age log-transformation revealed no outliers. We used variance inflation factor to test for collinearity of variables, and found none that

were collinear. Multiple imputations were used to handle missing data. Continuous variables are reported as mean ± SD, and categorical variables are reported as counts (%). Chi-square tests

and independent sample t-tests were used to compare brain tumor survivors and controls for categorical and continuous variables, respectively. We used Spearman’s test to assess the

correlation of adiposity measures with BMI and with each other in this study. To assess the association of adiposity with brain tumor status, we used binary logistic regression. The

dependent variable (event) was the cancer case status, with 56 events included in the analysis and 106 controls (non-events). Age, sex, %FM, WHR, and WHtR were included as the predictor

variables in the analysis. We rescaled the WHR and WHtR coefficients by multiplying the log-transformed data by 1064,65. Logistic regression was conducted based on the assumption that ten

events per predictor variable are needed for the analysis. As there are five predictor variables included in the analysis, our study is sufficiently powered to answer the main study

question. To explore the determinants of the adiposity patterns in CBT and controls, we performed exploratory subgroup analyses of the cancer cases and controls separately with multivariate

linear regression analysis. The dependent variables included %FM, WHR, and WHtR. The predictor variables of interest in CBT included age, sex, puberty, brain tumor histopathology, tumor

location, and treatments including surgery, radiotherapy, chemotherapy, and steroids. In addition, we included lifestyle factors encompassing diet, physical activity, screen time, and sleep

duration in the analysis. For controls, we included age, sex, puberty, diet, physical activity, screen time, and sleep duration in the analysis. The sample size of 56 events and 106

non-events provide adequate power for this analysis, as two events per variable are required in linear regression analyses to address the question of adiposity determinants in CBT and

controls66. To analyze the dietary patterns in participants, we used principal component analysis. Twenty-two food items were included in the factor analysis. The number of dietary patterns

retained was determined by visual inspection of scree plots in conjunction with eigenvalues (>1.0) and principal component interpretability. The factors were orthogonally transformed by

using the varimax rotation to ensure the independence of factors in the structure. Dietary patterns were characterized based on dietary items with their factor loadings ≥|0.30|. The PCA

scores for each pattern obtained for each individual represented how closely their food choices reflected one of the empirically-derived dietary patterns, with higher scores reflecting a

greater degree of adherence to that dietary pattern67. ADDITIONAL INFORMATION HOW TO CITE THIS ARTICLE: Wang, K.-W. _et al_. Adiposity in childhood brain tumors: A report from the Canadian

Study of Determinants of Endometabolic Health in Children (CanDECIDE Study). _Sci. Rep._ 7, 45078; doi: 10.1038/srep45078 (2017). PUBLISHER'S NOTE: Springer Nature remains neutral with

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Questionnaires and Dietary Pattern Analysis in 4 Ethnically Diverse Birth Cohorts. _The Journal of nutrition_, doi: 10.3945/jn.116.236729 (2016). Download references ACKNOWLEDGEMENTS We

thank the children and families who participated in the study, and the staff who helped with recruitment. We also thank Ms. Pei-Wen Wang for editorial assistance. M.C.S. was funded by the

Hamilton Health Sciences and Foundation, and the Pediatric Oncology Group of Ontario Research Unit. K.W.W. was funded by the Ontario Graduate Scholarship Program, and the Canadian Institutes

of Health Research (CIHR) Canada Graduate Scholarship-Master’s. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Medical Sciences Program, McMaster University, Hamilton, Ontario, Canada

Kuan-Wen Wang, Russell J. de Souza, Adam Fleming, Lehana Thabane & M. Constantine Samaan * Department of Pediatrics, McMaster University, Hamilton, Ontario, Canada Kuan-Wen Wang, Adam

Fleming, Katrin Scheinemann & M. Constantine Samaan * Division of Pediatric Endocrinology, McMaster Children’s Hospital, Hamilton, Ontario, Canada Kuan-Wen Wang & M. Constantine

Samaan * Department of Health Research Methods, Evidence and Impact, McMaster University, Hamilton, Ontario, Canada Russell J. de Souza, Lehana Thabane & M. Constantine Samaan * Division

of Pediatric Hematology/Oncology, McMaster Children’s Hospital, Hamilton, Ontario, Canada Adam Fleming * Division of Neurosurgery, Department of Surgery, McMaster Children’s Hospital,

Hamilton, Ontario, Canada Sheila K. Singh * McMaster Stem Cell and Cancer Research Institute, McMaster University, Hamilton, Ontario, Canada Sheila K. Singh * Division of Pediatric

Hematology/Oncology, Children’s Hospital of Eastern Ontario, Ottawa, Ontario, Canada Donna L. Johnston * Pediatric Hematology Oncology, London Health Sciences Center, London, Ontario, Canada

Shayna M. Zelcer * Division of Pediatric Hematology/Oncology/BMT, Department of Pediatrics, British Columbia Children’s Hospital, Vancouver, BC, Canada Shahrad Rod Rassekh * Division of

Orthopedic Surgery, Department of Surgery, McMaster University Medical Centre, Hamilton, Ontario, Canada Sarah Burrow * Division of Hematology/Oncology, University Children’s Hospital,

Basel, Switzerland Katrin Scheinemann * Department of Anesthesia, McMaster University, Hamilton, Ontario, Canada Lehana Thabane * Centre for Evaluation of Medicines, St. Joseph’s Health

Care, Hamilton, ON, Canada Lehana Thabane * Biostatistics Unit, St. Joseph’s Healthcare-Hamilton, Ontario, Canada Lehana Thabane Authors * Kuan-Wen Wang View author publications You can also

search for this author inPubMed Google Scholar * Russell J. de Souza View author publications You can also search for this author inPubMed Google Scholar * Adam Fleming View author

publications You can also search for this author inPubMed Google Scholar * Sheila K. Singh View author publications You can also search for this author inPubMed Google Scholar * Donna L.

Johnston View author publications You can also search for this author inPubMed Google Scholar * Shayna M. Zelcer View author publications You can also search for this author inPubMed Google

Scholar * Shahrad Rod Rassekh View author publications You can also search for this author inPubMed Google Scholar * Sarah Burrow View author publications You can also search for this author

inPubMed Google Scholar * Katrin Scheinemann View author publications You can also search for this author inPubMed Google Scholar * Lehana Thabane View author publications You can also

search for this author inPubMed Google Scholar * M. Constantine Samaan View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS M.C.S. is the

guarantor. Study conception and design determined by M.C.S., K.W.W., R.J.d.S., A.F., S.K.S., D.L.J., S.M.Z., S.R.R., S.B., K.S. and L.T. Subjects recruitment and data collection was done by

K.W.W., with the support from M.C.S, A.F., S.K.S., S.B., and K.S. Dietary data interpretation and analysis was completed by K.W.W., R.J.d.S., and M.C.S. Other statistical analyses and data

interpretation was completed by K.W.W., M.C.S., R.J.d.S., A.F., S.K.S., D.L.J., S.M.Z., S.R.R., S.B., K.S., and L.T. K.W.W. and M.C.S. drafted the manuscript. All authors provided critical

revisions of the manuscript and approved the final submitted version. CORRESPONDING AUTHOR Correspondence to M. Constantine Samaan. ETHICS DECLARATIONS COMPETING INTERESTS In the last five

years, Dr. de Souza has served as an external resource person to the World Health Organization’s Nutrition Guidelines Advisory Group on trans fats and saturated fats. The WHO paid for his

travel and accommodation to attend meetings from 2012–2015 to present and discuss this work. He has also done contract research for the Canadian Institutes of Health Research’s Institute of

Nutrition, Metabolism, and Diabetes, Health Canada, and the World Health Organization for which he received remuneration. He has held a grant from the Canadian Foundation for Dietetic

Research as a principal investigator, and is a co-investigator on several funded team grants from Canadian Institutes of Health Research. The other authors declare no conflict of interest.

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report from the Canadian Study of Determinants of Endometabolic Health in Children (CanDECIDE Study). _Sci Rep_ 7, 45078 (2017). https://doi.org/10.1038/srep45078 Download citation *

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