Sleep it off | Nature Reviews Neuroscience

Access through your institution Buy or subscribe To dissect the effects of increased sleep need from time spent awake, the authors used sleep-deprived mice and ‘sleepy’ mice, which carry a

mutation in the gene encoding salt-inducible kinase 3 (SIK3), an AMP-activated protein kinase (AMPK) family member. These _Sik3__Slp_/+ mice spend less time awake than do wild-type mice,

reflecting a consistently high sleep need. The authors analysed proteomic and phosphoproteomic data from whole-brain lysates and used these to make three group comparisons: _Sik3__Slp_/+

mice versus wild-type mice; mice that had been sleep-deprived for 6 hours versus mice that slept for 6 hours; and sleep-deprived mice versus mice that were sleep-deprived for 6 hours and

then had 3 hours of recovery sleep. Whereas the levels of most proteins did not seem to be altered with sleep need, there were considerable changes in the levels of different phosphoproteins

between the _Sik3__Slp_/+ mice and wild-type mice, and between the sleep-deprived and slept or recovery-slept mice. Changes in the levels of 329 phosphopeptides were common to all three

comparisons, suggesting that increased sleep need may be accompanied by changes in phosphorylation in the brain. This is a preview of subscription content, access via your institution ACCESS

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FAQs * Contact customer support REFERENCES ORIGINAL ARTICLE * Wang, Z. et al. Quantitative phosphoproteomic analysis of the molecular substrates of sleep. _Nature_

https://doi.org/10.1038/s41586-018-0218-8 (2018) Article  PubMed  PubMed Central  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Nature Reviews

Neuroscience http://www.nature.com/nrn/ Natasha Bray Authors * Natasha Bray View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR

Correspondence to Natasha Bray. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Bray, N. Sleep it off. _Nat Rev Neurosci_ 19, 444 (2018).

https://doi.org/10.1038/s41583-018-0037-9 Download citation * Published: 26 June 2018 * Issue Date: August 2018 * DOI: https://doi.org/10.1038/s41583-018-0037-9 SHARE THIS ARTICLE Anyone you

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