The capability of neonatal leukocytes to produce il-6 on stimulation assessed by whole blood culture

ABSTRACT ABSTRACT: IL-6 is a cytokine with a wide variety of influences on the cells involved in immune and inflammatory responses. Defective production of IL-6 may be partly responsible for

the impaired immune defense and inflammatory response often observed in the neonatal period. In our study, we used whole blood culture to examine the capacity of neonatal leukocytes to

produce IL-6 in response to various stimuli. IL-6 activity was evaluated by growth promoting assay using an IL-6-dependent murine hybrid-oma clone. IL-6 activity was undetectable in fresh or

unstimulated blood obtained from both newborns and adults. In contrast, incubation of whole blood with lipo-polysaccharide or concanavalin A resulted in marked IL-6 activity. After

stimulation, IL-6 activity was induced as early as 2 h after culture and increased with time, reaching a plateau at around 12 h. Comparative examinations suggested that the IL-6 activity

induced in neonatal blood on stimulation was similar to that seen in stimulated adult blood. Neutralization experiments with anti-IL-6 anti-serum confirmed the presence of IL-6 proteins in

the stimulated blood, and induction of cellular IL-6 mRNA was demonstrated in the stimulated blood as well. In addition, immunocytochemical observations suggested that the major IL-6

producing cells in the stimulated blood may be monocytes. The results suggest that the production of IL-6 in response to specified stimuli is normal at birth. SIMILAR CONTENT BEING VIEWED BY

OTHERS GROUP B STREPTOCOCCI INFECTION MODEL SHOWS DECREASED REGULATORY CAPACITY OF CORD BLOOD CELLS Article Open access 14 February 2022 WHOLE BLOOD BIOPHYSICAL IMMUNE PROFILING OF NEWBORN

INFANTS CORRELATES WITH IMMUNE RESPONSES Article Open access 31 March 2025 AN IL-10/DEL-1 AXIS SUPPORTS GRANULOPOIESIS AND SURVIVAL FROM SEPSIS IN EARLY LIFE Article Open access 23 January

2024 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, School of Medicine, Kanazawa University, Kanazawa, Ishikawa, 920, Japan Akihiro Yachie, Nobuhiko

Takano, Tohru Yokoi, Kimitaka Kato, Yoshihito Kasahara, Toshio Miyawaki & Noboru Taniguchi Authors * Akihiro Yachie View author publications You can also search for this author inPubMed 

Google Scholar * Nobuhiko Takano View author publications You can also search for this author inPubMed Google Scholar * Tohru Yokoi View author publications You can also search for this

author inPubMed Google Scholar * Kimitaka Kato View author publications You can also search for this author inPubMed Google Scholar * Yoshihito Kasahara View author publications You can also

search for this author inPubMed Google Scholar * Toshio Miyawaki View author publications You can also search for this author inPubMed Google Scholar * Noboru Taniguchi View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yachie, A., Takano, N., Yokoi,

T. _et al._ The Capability of Neonatal Leukocytes to Produce IL-6 on Stimulation Assessed by Whole Blood Culture. _Pediatr Res_ 27, 227–233 (1990).

https://doi.org/10.1203/00006450-199003000-00005 Download citation * Received: 21 July 1989 * Accepted: 08 November 1989 * Issue Date: 01 March 1990 * DOI:

https://doi.org/10.1203/00006450-199003000-00005 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is

not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative