Analysis of cerebrospinal fluid (csf) from neonates using proton magnetic resonance spectroscopy (mrs)


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ABSTRACT Samples of CSF from 13 full-terra newborn infants were studied using high resolution proton MRS. CSF (0.5mls) was stored at -70°C, freeze-dried, reconstituted in deuterium oxide and


a marker added for quantitation and chemical shift referencing. Spectra were obtained at 300 MHz, using presaturation to suppress the water signal. Ten samples were from neurologically


normal infants. Spectra showed peaks attributed to lactate, acetate, citrate, glutamine, glucose, alanine, 3-hydroxybutyrate, creatine, formate and inositol. Spectra from the CSF of 3


infants with post-asphyxial encephalopathy and seizures showed a peak attributed to the drug carrier propylene glycol and another peak representing millimolar concentrations of unknown


compound. Quantitative differences in the CSF spectra also occurred following asphyxia. Non-selective assay of multiple metabolites using proton MRS may help to define CSF markers of


cerebral ischaemia which would be suitable for conventional analysis. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Dept of Paediatrics, John Radcliffe Hospital and Dept of


Biochemistry, University of Oxford, Oxford, England Jonathan M Grigg, Peter N Kllby, Peter L Hope & George K Radda Authors * Jonathan M Grigg View author publications You can also search


for this author inPubMed Google Scholar * Peter N Kllby View author publications You can also search for this author inPubMed Google Scholar * Peter L Hope View author publications You can


also search for this author inPubMed Google Scholar * George K Radda View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and


permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Grigg, J., Kllby, P., Hope, P. _et al._ ANALYSIS OF CEREBROSPINAL FLUID (CSF) FROM NEONATES USING PROTON MAGNETIC RESONANCE SPECTROSCOPY


(MRS). _Pediatr Res_ 26, 506 (1989). https://doi.org/10.1203/00006450-198911000-00044 Download citation * Issue Date: 01 November 1989 * DOI: https://doi.org/10.1203/00006450-198911000-00044


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