Automated screening for mutants affecting dopaminergic-neuron specification in c. Elegans

ABSTRACT We describe an automated method to isolate mutant _Caenorhabditis elegans_ that do not appropriately execute cellular differentiation programs. We used a fluorescence-activated

sorting mechanism implemented in the COPAS Biosort machine to isolate mutants with subtle alterations in the cellular specificity of GFP expression. This methodology is considerably more

efficient than comparable manual screens and enabled us to isolate mutants in which dopamine neurons do not differentiate appropriately. Access through your institution Buy or subscribe This

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DEVELOPMENTAL GENE EXPRESSION ATLAS UNVEILS DIMORPHIC GENE NETWORKS IN _C. ELEGANS_ Article Open access 20 May 2024 A FULL-BODY TRANSCRIPTION FACTOR EXPRESSION ATLAS WITH COMPLETELY RESOLVED

CELL IDENTITIES IN _C. ELEGANS_ Article Open access 09 January 2024 DISSECTING THE GENETIC LANDSCAPE OF GPCR SIGNALING THROUGH PHENOTYPIC PROFILING IN _C. ELEGANS_ Article Open access 18

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Article  CAS  PubMed  PubMed Central  Google Scholar  Download references ACKNOWLEDGEMENTS We thank A. Kruyer, A. Pretorian, J. Recio and Q. Chen for technical assistance, S. Sarin for

calculating the degree of saturation, V. Bertrand and F. Zhang for communicating unpublished results, and S. Mitani (Tokyo Women's Medical University School of Medicine) for providing

_lin-32_ deletion alleles. This work was funded by the US National Institutes of Health (R01NS039996-05; R01NS050266-03), the Howard Hughes Medical Institute, an European Molecular Biology

Organization long-term fellowship to M.D. and N.F., and a Marie Curie Outgoing International fellowship to N.F. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry and

Molecular Biophysics, Howard Hughes Medical Institute, Columbia University Medical Center, 701 W. 168th Street, New York, 10032, New York, USA Maria Doitsidou, Nuria Flames, Albert C Lee, 

Alexander Boyanov & Oliver Hobert Authors * Maria Doitsidou View author publications You can also search for this author inPubMed Google Scholar * Nuria Flames View author publications

You can also search for this author inPubMed Google Scholar * Albert C Lee View author publications You can also search for this author inPubMed Google Scholar * Alexander Boyanov View

author publications You can also search for this author inPubMed Google Scholar * Oliver Hobert View author publications You can also search for this author inPubMed Google Scholar

CONTRIBUTIONS M.D. conducted the genetic screens (manual and sorter), implemented the sorter strategy, mapped and characterized mutants, and co-wrote the paper; N.F. implemented the sorter

strategy and conducted sorter screens; A.C.L. conducted manual screens; A.B. assisted in mapping mutants; and O.H. initiated and supervised the project, and co-wrote the paper. CORRESPONDING

AUTHORS Correspondence to Maria Doitsidou or Oliver Hobert. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–2, Supplementary Tables 1–6, Supplementary

Methods (PDF 3378 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Doitsidou, M., Flames, N., Lee, A. _et al._ Automated screening for mutants

affecting dopaminergic-neuron specification in _C. elegans_. _Nat Methods_ 5, 869–872 (2008). https://doi.org/10.1038/nmeth.1250 Download citation * Received: 28 April 2008 * Accepted: 29

July 2008 * Published: 31 August 2008 * Issue Date: October 2008 * DOI: https://doi.org/10.1038/nmeth.1250 SHARE THIS ARTICLE Anyone you share the following link with will be able to read

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