Reply to “lack of evidence for dna methylation of invader4 retroelements in drosophila and implications for dnmt2-mediated epigenetic regulation”

Access through your institution Buy or subscribe Phalke _et al_. reply: In their Correspondence regarding our work published in _Nature Genetics_1, Schaefer and Lyko argue that the

processive DNA methyltransferase activity in every dinucleotide context at _Invader4_ retroelements that we observed is difficult to reconcile with other reports about the activity of DNMT2

in _Drosophila_. However, until now, no other studies specifically analyzed DNA methylation at _Invader4_ retroelements. Using high through-put bisulfite sequencing of _Invader4_ long

terminal repeats (LTRs), Schaefer and Lyko could not detect significant DNA methylation in a wild-type _w__1118_ strain, which contradicts our published data showing early embryonic,

DNMT2-dependent DNA methylation at _Invader4_ LTRs. Schaefer and Lyko ultimately conclude that both the primers we used and the bisulfite treatment we performed in our analysis caused this

discrepancy. However, methodological differences do not explain why our experiments generated more complete conversion of cytosines in the _Dnmt2_-null mutant control and in older embryos as

compared to all the experiments reported by Schaefer and Lyko. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution

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Contact customer support REFERENCES * Phalke, S. et al. _Nat. Genet._ 41, 696–702 (2009). Article  CAS  Google Scholar  * Ryder, E. et al. _Genetics_ 177, 615–629 (2007). Article  CAS 

Google Scholar  Download references AUTHOR INFORMATION Author notes * Institute of Biology and Genetics, Martin Luther University Halle-Wittenberg, Halle, Germany. Present address: Institute

of Molecular and Cell Biology, 61 Biopolis Way, Singapore (S.P.). AUTHORS AND AFFILIATIONS * Gunter Reuter Authors * Sameer Phalke View author publications You can also search for this

author inPubMed Google Scholar * Olaf Nickel View author publications You can also search for this author inPubMed Google Scholar * Gunter Reuter View author publications You can also search

for this author inPubMed Google Scholar CONTRIBUTIONS S.P. and O.N. analyzed the data. G.R. conceived the study and wrote the manuscript. CORRESPONDING AUTHOR Correspondence to Gunter

Reuter. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Phalke, S., Nickel, O. & Reuter, G. Reply to “Lack of evidence for DNA methylation of _Invader4_ retroelements in _Drosophila_ and implications for Dnmt2-mediated epigenetic regulation”.

_Nat Genet_ 42, 921 (2010). https://doi.org/10.1038/ng1110-921 Download citation * Published: 27 October 2010 * Issue Date: November 2010 * DOI: https://doi.org/10.1038/ng1110-921 SHARE

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