Peripheral blood regulatory t cells in patients with diffuse systemic sclerosis (ssc) before and after autologous hematopoietic sct: a pilot study

ABSTRACT The present pilot study aims to evaluate the frequency and the function of regulatory T (Treg) cells in patients with diffuse cutaneous SSc (dcSSc) before and after autologous

hematopoietic SCT (aHSCT). Peripheral blood lymphocytes from seven dcSSc patients were analyzed before and 24 months after aHSCT and were compared with those from seven healthy donors

(controls). Immunophenotyping of CD4+CD25highFoxP3+ natural Treg (nTreg), CD4+CD25+TGF-β+ and CD4+CD25+IL-10+ adaptive Treg (aTreg) cell subsets was performed using four-color flow

cytometry. Treg-suppressive capability was measured after coculture with autologous T effector cells by evaluation of T-cell proliferation using 3H-thymidine incorporation. Peripheral

CD4+CD25highFoxP3+ (2±0.5 vs 4.2±1.1, _P_<0.01), CD4+CD25+TGF-β+ (6.9±1.8 vs 14.6±5.0, _P_<0.05) and CD4+CD25+IL-10+ (10.7±0.5 vs 16.1±3.2, _P_<0.01) Tregs as well as

CD4+CD25highCD127low Tregs suppressive capacity (_P_<0.05) were decreased in dcSSc patients vs controls. After aHSCT (_n_=7), the percentages of CD4+CD25highFoxP3+ (4.1±1.8) and

CD4+CD25+IL-10+ (15.7±2.2) Treg cells and the suppressive activity of CD4+CD25highCD127low were restored to the levels in controls. The decreased frequency and the functional defect of

peripheral Treg cells from patients with dcSSc are reversed following aHSCT to reach those observed in controls. This pilot study brings evidence of an effective restoration of nTreg and

aTreg subsets, and recovery of nTreg suppressive function following aHSCT. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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AFTER ALLOGENEIC HEMATOPOIETIC STEM CELL TRANSPLANTATION Article 04 March 2021 CD70 EXPRESSION DETERMINES THE THERAPEUTIC EFFICACY OF EXPANDED HUMAN REGULATORY T CELLS Article Open access 14

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acknowledge the Association des Sclérodermiques de France (ASF) and the Groupe Francophone de Recherche sur la Sclérodermie (GFRS). This work was supported by grants from the Groupe Français

de Recherche sur la Sclérodermie (GFRS). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * INSERM U976 and Université Paris Diderot Sorbonne Paris Cité, Hôpital Saint-Louis, Paris, France J

Baraut, E I Grigore, F Jean-Louis, D Farge & L Michel * Service de Médecine Interne, Assistance Publique-Hôpitaux de Paris, Hôpital Saint-Louis, Paris, France S H Khelifa & D Farge *

Service de Médecine Interne, Hôpital Hôtel Dieu, Nantes, France C Durand * INSERM U957 and Université Nantes Atlantique, Nantes, France F Verrecchia Authors * J Baraut View author

publications You can also search for this author inPubMed Google Scholar * E I Grigore View author publications You can also search for this author inPubMed Google Scholar * F Jean-Louis

View author publications You can also search for this author inPubMed Google Scholar * S H Khelifa View author publications You can also search for this author inPubMed Google Scholar * C

Durand View author publications You can also search for this author inPubMed Google Scholar * F Verrecchia View author publications You can also search for this author inPubMed Google

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Scholar CORRESPONDING AUTHOR Correspondence to L Michel. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no conflict of interest. RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Baraut, J., Grigore, E., Jean-Louis, F. _et al._ Peripheral blood regulatory T cells in patients with diffuse systemic sclerosis (SSc) before and after

autologous hematopoietic SCT: a pilot study. _Bone Marrow Transplant_ 49, 349–354 (2014). https://doi.org/10.1038/bmt.2013.202 Download citation * Received: 13 August 2012 * Revised: 26

September 2013 * Accepted: 10 October 2013 * Published: 23 December 2013 * Issue Date: March 2014 * DOI: https://doi.org/10.1038/bmt.2013.202 SHARE THIS ARTICLE Anyone you share the

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Nature SharedIt content-sharing initiative KEYWORDS * systemic sclerosis * regulatory T cells * hematopoietic stem cell transplantation * T-cell proliferation