Heterogeneity in radiation sensitivity within human primary tumour cell cultures as detected by the sce assay

ABSTRACT The ability of the sister chromatid exchange (SCE) assay to detect heterogeneity in intrinsic radiation sensitivity was investigated. In order to identify tumour cell

subpopulations, frequency histograms of cis-diamminedichloroplatinum (II) (cPt)-induced SCEs were generated and compared to those from cultures that had been irradiated 96 h before drug

treatment. The results suggested that subpopulations with different radiosensitivities were present in nine of 18 human primary tumour cell cultures evaluated. When the effects of prior

irradiation on the subsequent X-ray survival response and on cPt-induced SCE frequency histograms were compared, a good correlation was obtained between the two assays regarding the

prediction of heterogeneity in radioresponse. These results suggest that primary cultures can contain both radiation-sensitive and radiation-resistant cells, and thus heterogeneity in

intrinsic radiosensitivity may exist in human solid tumours. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS

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institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS IMPACT OF FRACTIONATED CISPLATIN AND RADIATION TREATMENT ON CELL GROWTH AND

ACCUMULATION OF DNA DAMAGE IN TWO NORMAL CELL TYPES DIFFERING IN ORIGIN Article Open access 09 September 2023 DATASETS OF _IN VITRO_ CLONOGENIC ASSAYS SHOWING LOW DOSE HYPER-RADIOSENSITIVITY

AND INDUCED RADIORESISTANCE Article Open access 08 September 2022 IONIZING RADIATION RESULTS IN A MIXTURE OF CELLULAR OUTCOMES INCLUDING MITOTIC CATASTROPHE, SENESCENCE, METHUOSIS, AND

IRON-DEPENDENT CELL DEATH Article Open access 23 November 2020 AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Experimental Radiotherapy, University of Texas M.D. Anderson

Hospital, Houston, 77030 PJ Tofilon Authors * PJ Tofilon View author publications You can also search for this author inPubMed Google Scholar * CM Vines View author publications You can also

search for this author inPubMed Google Scholar * RE Meyn View author publications You can also search for this author inPubMed Google Scholar * J Wike View author publications You can also

search for this author inPubMed Google Scholar * WA Brock View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions

ABOUT THIS ARTICLE CITE THIS ARTICLE Tofilon, P., Vines, C., Meyn, R. _et al._ Heterogeneity in radiation sensitivity within human primary tumour cell cultures as detected by the SCE assay.

_Br J Cancer_ 59, 54–60 (1989). https://doi.org/10.1038/bjc.1989.11 Download citation * Issue Date: 01 January 1989 * DOI: https://doi.org/10.1038/bjc.1989.11 SHARE THIS ARTICLE Anyone you

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