Isolation of uv-resistant revertants from a xeroderma pigmentosum complementation group a cell line

ABSTRACT Xeroderma pigmentosum (XP) is an autosomal recessive disease. Cells cultured from XP patients are hypersensitive to the lethal effects of UV light1–6. Most XP cells are defective in

an early stage in DNA repair of UV light-induced damage. The nature of the genetic defect of the XP syndrome has not been defined. To address this problem, we attempted to isolate

UV-resistant cells from a cell line derived from an XP complementation group A (XPA) patient. By using a selection scheme capable of detecting one UV-resistant cell in a population of 108

cells, several UV-resistant clones were isolated at frequencies between 1 × 10−7 and 2 × 10−8. Here we describe the isolation and initial characterization of these phenotypic revertants.

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Department of Haematology, Children's Hospital, Boston, Massachusetts, 02115, USA AUTHORS AND AFFILIATIONS * Department of Pathology, Dana–Farber Cancer Institute, Harvard Medical

School, Boston, Massachusetts, USA Brigitte Royer-Pokora & William A. Haseltine Authors * Brigitte Royer-Pokora View author publications You can also search for this author inPubMed 

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CITE THIS ARTICLE Royer-Pokora, B., Haseltine, W. Isolation of UV-resistant revertants from a xeroderma pigmentosum complementation group A cell line. _Nature_ 311, 390–392 (1984).

https://doi.org/10.1038/311390a0 Download citation * Received: 12 June 1984 * Accepted: 10 July 1984 * Issue Date: 27 September 1984 * DOI: https://doi.org/10.1038/311390a0 SHARE THIS

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