Targeting to cells of fluorescent liposomes covalently coupled with monoclonal antibody or protein a

ABSTRACT Many applications envisioned for liposomes in cell biology and chemotherapy require their direction to specific cellular targets1–3. The ability to use antibody as a means of

conferring specificity to liposomes would markedly increase their usefulness. We report here a method for covalently coupling soluble proteins, including monoclonal antibody and

_Staphylococcus aureus_ protein A (ref. 4), to small sonicated liposomes, by using the heterobifunctional cross-linking reagent _N_-hydroxysuccinimidyl 3-(2-pyridyldithio)propionate (SPDP,

Pharmacia). Liposomes bearing covalently coupled mouse monoclonal antibody against human _β_2-microglobulin [antibody B1.1G6 (IgG2a, _κ_) (B. Malissen _et al._, in preparation)] bound

specifically to human, but not to mouse cells. Liposomes bearing protein A became bound to human cells previously incubated with the B1.1G6 antibody, but not to cells incubated without

antibody. The coupling method results in efficient binding of protein to the liposomes without aggregation and without denaturation of the coupled ligand; at least 60% of liposomes bound

functional protein. Further, liposomes did not leak encapsulated carboxyfluorescein (CF) as a consequence of the reaction. Access through your institution Buy or subscribe This is a preview

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LIPOSOMES BY SELF-ASSEMBLY Article 19 February 2024 NON-SPECIFIC INTERACTIONS OF ANTIBODY-OLIGONUCLEOTIDE CONJUGATES WITH LIVING CELLS Article Open access 15 March 2021 COACERVATE VESICLES

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AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Centre d'Immunologie INSERM-CNRS de Marseille-Luminy, Case 906, 13288, Marseille, Cedex 2, France Lee D. Leserman, Jacques Barbet & 

François Kourilsky * Laboratory of Theoretical Biology, National Cancer Institute, National Institutes of Health, Bethesda, Maryland, 20205 John N. Weinstein Authors * Lee D. Leserman View

author publications You can also search for this author inPubMed Google Scholar * Jacques Barbet View author publications You can also search for this author inPubMed Google Scholar *

François Kourilsky View author publications You can also search for this author inPubMed Google Scholar * John N. Weinstein View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Leserman, L., Barbet, J., Kourilsky, F. _et al._ Targeting to cells of

fluorescent liposomes covalently coupled with monoclonal antibody or protein A. _Nature_ 288, 602–604 (1980). https://doi.org/10.1038/288602a0 Download citation * Received: 14 July 1980 *

Accepted: 17 October 1980 * Issue Date: 11 December 1980 * DOI: https://doi.org/10.1038/288602a0 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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