Use of tetrazolium compounds in oxidative enzyme histo- and cyto- chemistry

ABSTRACT A NEW tetrazolium salt previously synthesized has been evaluated for its ability to overcome the technical and theoretical barriers of either the MTT-Co (ref. 1) or nitro-BT

methods2,3. This compound is TNBT or 2,2′,5-5′-tetra-_p_-nitrophenyl-3,3′ -(3,3′ -dimethoxy-4,4′-biphenylene)-ditetrazolium chloride (Fig. 1), the formazan of which is purplish black4. The

TNBT used in the following investigations produced a brownish-black precipitate in tissue sections. Though quite low in water solubility, minimal concentrations of TNBT are required for the

effective demonstration of the succinic dehydrogenase system in fresh, frozen sections of tissue of mammalian tissues or human vaginal smear preparations. The following were investigated in

mouse myocardium and kidney: (1) effect of concentration of dye on the products of TNBT formazan in slices of these tissues; (2) effect of _p_H. on the reduction of TNBT by the succinic

dehydrogenase system in tissue sections in the presence or absence of appropriate substrate; (3) solubility of TNBT formazan in aqueous, alcoholic and other organic solvents necessary for

the effective resin-mounting of sections of tissue containing precipitates of formazan. Access through your institution Buy or subscribe This is a preview of subscription content, access via

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ALDEHYDE-TREATED BIOLOGICAL TISSUES Article Open access 03 December 2020 FLUORESCENT PROBES FOR MONITORING MYELOPEROXIDASE-DERIVED HYPOCHLOROUS ACID: A COMPARATIVE STUDY Article Open access

03 June 2022 OXIDATION OF ETHIDIUM-BASED PROBES BY BIOLOGICAL RADICALS: MECHANISM, KINETICS AND IMPLICATIONS FOR THE DETECTION OF SUPEROXIDE Article Open access 29 October 2020 REFERENCES *

Pearse, A. G. E., _Histochemistry, Theoretical and Applied_, second ed. (J. and A. Churchill, London, 1959). Google Scholar  * Nachlas, M. M., Tsou, K. C., DeSouza, E., Cheng, C. S., and

Seligman, A. M., _J. Histochem. and Cytochem._, 5, 420 (1957). Article  CAS  Google Scholar  * Nachlas, M. M., Walker, D. G., and Seligman, A. M., _J. Biophys. Biochem. Cytol._, 4, 29

(1958). Article  CAS  Google Scholar  * Tsou, K. C., Cheng, C. S., Nachlas, M. M., and Seligman, A. M., _J. Amer. Chem. Soc._, 78, 6139 (1956). Article  CAS  Google Scholar  * Sedar, A. W.,

and Rosa, C. G., _J. Ultrastructure Res._, 5, 226 (1961). Article  CAS  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Daniel Baugh Institute of Anatomy,

Jefferson Medical College of Philadelphia, C. G. ROSA * Dajac Laboratories, Borden Chemical Co., Philadelphia KWAN-CHUNG TSOU Authors * C. G. ROSA View author publications You can also

search for this author inPubMed Google Scholar * KWAN-CHUNG TSOU View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE ROSA, C., TSOU, KC. Use of Tetrazolium Compounds in Oxidative Enzyme Histo- and Cyto- chemistry. _Nature_ 192, 990–991 (1961).

https://doi.org/10.1038/192990a0 Download citation * Issue Date: 09 December 1961 * DOI: https://doi.org/10.1038/192990a0 SHARE THIS ARTICLE Anyone you share the following link with will be

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