Glutamic–aspartic transaminase of pig heart muscle


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ABSTRACT THE glutamic–aspartic transaminase of pig heart muscle has been more than 98 per cent resolved into apoenzyme and co-factor by carrying out a differential heat inactivation in


phosphate buffer in the course of purification of the enzyme. The apoenzyme has been purified 64-fold, by relatively simple and reproducible techniques. The highest purification factor


previously reported1 for the apoenzyme is 26. The specific activity of the purified apoenzyme compares favourably with that of an electrophoretically homogeneous preparation of the


holoenzyme2. Cammarata and Cohen's suggestion3 that purification of the apoenzyme necessarily results in partial inactivation has been disproved, since it is possible to account for all


the enzyme activity among the various protein fractions obtained at each step in the purification. SIMILAR CONTENT BEING VIEWED BY OTHERS CRYSTAL STRUCTURES OF THE ELUSIVE _RHIZOBIUM ETLI_


L-ASPARAGINASE REVEAL A PECULIAR ACTIVE SITE Article Open access 18 November 2021 GENERALIZED ENZYMATIC MECHANISM OF CATALYSIS BY TETRAMERIC L-ASPARAGINASES FROM MESOPHILIC BACTERIA Article


Open access 15 October 2020 TRACING THE LACTATE SHUTTLE TO THE MITOCHONDRIAL RETICULUM Article Open access 08 September 2022 ARTICLE PDF REFERENCES * O'Kane and Gunsalus, _J. Biol.


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AFFILIATIONS * William Ramsay and Ralph Forster Laboratory, University College, Gower St., London, W.C.1. B. E. C. BANKS, K. G. OLDHAM, E. M. THAIN & C. A. VERNON Authors * B. E. C.


BANKS View author publications You can also search for this author inPubMed Google Scholar * K. G. OLDHAM View author publications You can also search for this author inPubMed Google Scholar


* E. M. THAIN View author publications You can also search for this author inPubMed Google Scholar * C. A. VERNON View author publications You can also search for this author inPubMed 


Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE BANKS, B., OLDHAM, K., THAIN, E. _et al._ Glutamic–Aspartic Transaminase of Pig Heart


Muscle. _Nature_ 183, 1187 (1959). https://doi.org/10.1038/1831187a0 Download citation * Issue Date: 25 April 1959 * DOI: https://doi.org/10.1038/1831187a0 SHARE THIS ARTICLE Anyone you


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