Prb2/p130 target genes in non-small lung cancer cells identified by microarray analysis

ABSTRACT The retinoblastoma gene family consisting of _RB/p105_, _p107_, and _RB2/p130_ cooperate to regulate cell-cycle progression through the G1 phase of the cell cycle. Previous data

demonstrated an independent role for the reduction or loss of pRb2/p130 expression in the formation and/or progression of lung carcinoma. Rb2/p130 is mutated in a human cell line of lung

small cell carcinoma as well as in primary lung tumors. To identify potential pRb2/p130 target genes in an unbiased manner, we have utilized an adenovirus-mediated expression system of

pRb2/p130 in a non-small lung cancer cell line to identify specific genes that are regulated by pRb2/p130. Using oligonucleotide arrays, a number of Rb2/p130 downregulated genes were

identified and their regulation was confirmed by semiquantitative reverse transcription–polymerase chain reaction (RT–PCR) and Western blot analysis. As a result, 40 genes showed greater

than 2.0-fold modification in their expression level after the _RB2/p130_ viral transduction. In conclusion, coupling adenoviral overexpression with microarray and semiquantitative RT–PCR

analyses proved to be a versatile strategy for identifying pRb2/p130 target genes and for better understanding the expression profiles of these genes. Our results may also contribute to

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_Science_, 271, 1595–1597. Download references ACKNOWLEDGEMENTS This work was supported by NIH grants and the Sbarro Health Organization to AG. G.R. acknowledges the Ph.D. program:

“Diagnostic, quantitative, and molecular pathology” of the University of Siena, Italy. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Sbarro Institute for Cancer Research and Molecular

Medicine, College of Science and Technology, Temple University, Philadelphia, 19122, PA, USA Giuseppe Russo, Pier Paolo Claudio, Peter Stiegler, Marcella Macaluso & Antonio Giordano *

Institute of Human Pathology and Oncology, University of Siena, Italy Giuseppe Russo * Dipartimento di Scienze Odontostomatologiche e Maxillo-Facciali, Universita' di Napoli ‘Federico

II’, Italy Pier Paolo Claudio * Department of Pathology, Anatomy and Cell Biology, Thomas Jefferson University, Philadelphia, 19107, PA, USA Yan Fu * Jefferson Medical College, KCI Room 376,

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Russo, G., Claudio, P., Fu, Y. _et al._ pRB2/p130 target genes in non-small lung cancer cells identified by microarray analysis. _Oncogene_ 22, 6959–6969 (2003).

https://doi.org/10.1038/sj.onc.1206866 Download citation * Received: 07 April 2003 * Revised: 12 June 2003 * Accepted: 13 June 2003 * Published: 09 October 2003 * Issue Date: 09 October 2003

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not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * lung cancer * Rb2/p130 * microarray analysis