Involvement of aquaporins in colorectal carcinogenesis

ABSTRACT Aquaporins (AQPs) are important in controlling water permeability. As AQP1 is known as a serum-responsive gene, we hypothesized that AQP expression may be involved in the

development of human cancer. By reverse transcriptase–polymerase chain reaction analysis, expression of AQPs 1, 3, and 5 was found in seven colon and colorectal cancer cell lines. Western

blot analysis confirmed their expression in four of these cell lines. _In situ_ hybridization demonstrated that during colorectal carcinogenesis, the expression of AQPs 1 and 5 was induced

in early-stage disease (early dysplasia) and maintained through the late stages of colon cancer development. Expression of AQPs 1 and 5 was maintained even in metastatic lesions in the

liver. These findings demonstrate that the expression of several AQPs is found in tumor cells and is associated with an early stage of colorectal cancer development. These novel observations

suggest that multiple AQP expression may be advantageous to tumorigenesis, which may lead to a better understanding of colorectal carcinogenesis. Access through your institution Buy or

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AQUAPORIN WATER CHANNELS: ROLES BEYOND RENAL WATER HANDLING Article 17 July 2023 SLC26A9 PROMOTES COLORECTAL TUMORIGENESIS BY MODULATING WNT/Β-CATENIN SIGNALING Article Open access 09 March

2024 DECREASED NHE3 EXPRESSION IN COLON CANCER IS ASSOCIATED WITH DNA DAMAGE, INCREASED INFLAMMATION AND TUMOR GROWTH Article Open access 30 August 2022 REFERENCES * Agre P, Bonhivers M and

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and Chen X . (2001). _FEBS Lett._, 489, 4–7. Download references ACKNOWLEDGEMENTS We thank Dr Li K Su, who provided total RNA for the three colon cell lines used in this study. The study was

supported in part by NIH/NCI Grant P50 CA96784-01 (to CM), American Cancer Society Grant RPG-98-054 (to LM), Fondation de France, AP-HP and Lilly Fondation Grant (to J-CS), Cancer Center

Grant P30 CA 16620 (to MD Anderson Cancer Center), and Tobacco Research Fund from State of Texas (to MD Anderson Cancer Center). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * The Head and

Neck Cancer Research Division, Department of Otolaryngology, The Johns Hopkins University School of Medicine, Baltimore, 21205, MD, USA Chulso Moon, Juna Lee, Mohammad Obaidul Hoque, Barry

Trink & David Sidransky * Department of Oncology and Sidney Kimmel Comprehensive Cancer Center, The Johns Hopkins University School of Medicine, Baltimore, 21205, MD, USA Chulso Moon 

& David Sidransky * Division of Cancer Medicine, Gustave Roussy Institute, 39 Rue Camille Desmoulins, Villejuif, 94805, France Jean-Charles Soria * Department of Thoracic/Head and Neck

Medical Oncology, Molecular Biology Laboratory, The University of Texas MD Anderson Cancer Center, Houston, 77030, TX, USA Se Jin Jang, Yoon Soo Chang & Li Mao * Pathology Department,

Hospital Tenon, AP-HP, 4 rue de la Chine, Paris, 75020, France Mathilde Sibony Authors * Chulso Moon View author publications You can also search for this author inPubMed Google Scholar *

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Correspondence to Chulso Moon. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Moon, C., Soria, JC., Jang, S. _et al._ Involvement of aquaporins in

colorectal carcinogenesis. _Oncogene_ 22, 6699–6703 (2003). https://doi.org/10.1038/sj.onc.1206762 Download citation * Received: 03 February 2003 * Revised: 29 April 2003 * Accepted: 06 May

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KEYWORDS * aquaporin * _in situ_ hybridization * RT–PCR * colon cancer * carcinogenesis