Protection of internal (ttaggg)n repeats in chinese hamster cells by telomeric protein trf1

ABSTRACT Chinese hamster cells have large interstitial (TTAGGG) bands (ITs) which are unstable and should be protected by an unknown mechanism. Here, we expressed in Chinese hamster V79

cells green fluorescent protein (GFP)-tagged human TRF1, and found that a major fraction of GFP-TRF1 bound to ITs is diffusionally mobile. This fraction strongly decreases after treatment of

cells with wortmannin, a protein kinase inhibitor, and this drug also increases the frequency of chromosome aberrations. Ionizing radiation does not induce detectable translocation of

GFP-TRF1 to the sites of random double-strand breaks visualized using antibodies against histone _γ_-H2AX. TRF1 is known to be eliminated from telomeres by overexpression of tankyrase 1

which induces TRF1 poly(ADP-ribosyl)ation. We transfected V79 cells by plasmid encoding tankyrase 1 and found that the frequency of chromosome rearrangements is increased in these cells

independently of their treatment by IR. Taken together, our results suggest that TRF1 is involved in sequence-specific protection of internal nontelomeric (TTAGGG)_n_ repeats. Access through

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BEING VIEWED BY OTHERS TELOMERE DAMAGE INDUCES INTERNAL LOOPS THAT GENERATE TELOMERIC CIRCLES Article Open access 20 October 2020 CHROMOSOME END PROTECTION BY RAP1-MEDIATED INHIBITION OF

DNA-PK Article Open access 16 April 2025 ZNF524 DIRECTLY INTERACTS WITH TELOMERIC DNA AND SUPPORTS TELOMERE INTEGRITY Article Open access 12 December 2023 ABBREVIATIONS * ATM: Ataxia

teleangiectasia-mutated protein * CA: chromosome aberration * DSB: double-strand DNA break * GFP: green fluorescent protein * IT: internal non-telomeric blocks of (TTAGGG)_n_ sequences *

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Zhong Z, Shiue L, Kaplan S and de Lange T . (1992). _Mol. Cell. Biol._, 12, 4834–4843. Download references ACKNOWLEDGEMENTS We thank S Smith for her kind gift of plasmids pTetFLNLS and

pUHR15-1. This research was supported by the Office of Science (BER), US Department of Energy, Grant No. DE-FG03-01ER63070, by the National Institutes of Health Grant No. 5R01-HD39830-02, by

the Deutsche Forschung Gemeinschaft Grant 436 RUS113/126/0, by the Civilian Research and Development Foundation Grant ST-012-0 and the Russian Fund for Basic Research Grants 01-04-49486 and

02-04-49145. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of Chromosome Stability, Institute of Cytology, Russian Academy of Sciences, 194064 St, Petersburg, Russia Raisa

Ivanovna Krutilina, Alexandra Nikolaevna Smirnova, Olga Stanislavovna Mudrak, Nadezhda Mikhailovna Pleskach, Maria Pavlovna Svetlova & Nikolai Viktorovich Tomilin * Institute of

Biochemistry, Free University of Berlin, Thielallee 63, Berlin-Dahlem, 14195, Germany Shiao-Li Oei * Department of Biological Chemistry, UC Davis School of Medicine, Davis, 95616, CA, USA

Peter M Yau, Edwin Morton Bradbury & Andrey Olegovich Zalensky * Bioscience Division, MSM 888, Los Alamos National Laboratory, Los Alamos, 87545, NM, USA Edwin Morton Bradbury Authors *

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Correspondence to Nikolai Viktorovich Tomilin. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Krutilina, R., Smirnova, A., Mudrak, O. _et al._

Protection of internal (TTAGGG)_n_ repeats in Chinese hamster cells by telomeric protein TRF1. _Oncogene_ 22, 6690–6698 (2003). https://doi.org/10.1038/sj.onc.1206745 Download citation *

Received: 24 January 2003 * Revised: 29 April 2003 * Accepted: 30 April 2003 * Published: 02 October 2003 * Issue Date: 02 October 2003 * DOI: https://doi.org/10.1038/sj.onc.1206745 SHARE

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clipboard Provided by the Springer Nature SharedIt content-sharing initiative KEYWORDS * internal telomeric repeats * instability * TRF1 * wortmannin * tankyrase 1