Vibration-based cell engineering

ABSTRACT Cell engineering has the aim of producing cells with controlled phenotype for medical use, for example, for cell therapy, cell transplantation or drug discovery. However, chemical

induction of cell phenotypes, in particular, the use of inductive media and growth factors, often lacks specificity, might be unsuitable for clinical use and remains costly and difficult to

scale up. Alternatively, mechanotransductive stimulation can be applied to engineer cells with specific phenotypes. In this Review, we discuss vibration as a mechanotransductive

cell-engineering tool for both in vitro phenotypic control and in vivo regenerative therapy. We examine how vibration devices can be designed to provide specific frequencies and amplitudes

to which cells respond through either adhesion-induced or channel-induced mechanotransduction pathways. We further highlight key applications of vibrational stimulation for bone regeneration

as well as whole-body vibration as regenerative therapy, identifying important mechanisms of action and gaps in translational pipelines. KEY POINTS * Cells can respond to their mechanical

environment through phenotypic and functional changes. * Vibration is being explored for cell engineering to modify cellular growth, motility and differentiation. * Various vibration

parameters (frequency, amplitude and duration) are being explored for cell engineering, but their relation to mechanotransductive signalling and phenotypic changes are yet to be fully

understood. * Vibrational stimulation might be clinically applied for cell therapy or regenerative medicine, but scalibility and optimization of parameters remain challenging. Access through

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SIMILAR CONTENT BEING VIEWED BY OTHERS ULTRASOUND-ASSISTED TISSUE ENGINEERING Article 02 April 2024 EFFECTS OF EXTRACELLULAR MATRIX VISCOELASTICITY ON CELLULAR BEHAVIOUR Article 26 August

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for grants EP/N013905/1 and EP/P001114/1. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Centre for the Cellular Microenvironment (CeMi), Department of Biomedical Engineering, University of

Strathclyde, Glasgow, UK Olivia Johnson-Love, Stuart Reid & Peter G. Childs * Centre for the Cellular Microenvironment (CeMi), University of Glasgow, Glasgow, UK Manuel Salmeron-Sanchez 

& Matthew J. Dalby Authors * Olivia Johnson-Love View author publications You can also search for this author inPubMed Google Scholar * Manuel Salmeron-Sanchez View author publications

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publications You can also search for this author inPubMed Google Scholar * Matthew J. Dalby View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS

O.J.-L., P.G.C. and M.J.D. led the writing of the review, with all authors involved in contributing to different sections. All authors critically read the review. CORRESPONDING AUTHORS

Correspondence to Peter G. Childs or Matthew J. Dalby. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests. PEER REVIEW PEER REVIEW INFORMATION _Nature Reviews

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