An online resource for gpcr structure determination and analysis

ABSTRACT G-protein-coupled receptors (GPCRs) transduce physiological and sensory stimuli into appropriate cellular responses and mediate the actions of one-third of drugs. GPCR structural

studies have revealed the general bases of receptor activation, signaling, drug action and allosteric modulation, but so far cover only 13% of nonolfactory receptors. We broadly surveyed the

receptor modifications/engineering and methods used to produce all available GPCR crystal and cryo-electron microscopy (cryo-EM) structures, and present an interactive resource integrated

in GPCRdb (http://www.gpcrdb.org) to assist users in designing constructs and browsing appropriate experimental conditions for structure studies. Access through your institution Buy or

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ANALYSIS PLATFORM Article 10 November 2021 ALPHAFOLD3 VERSUS EXPERIMENTAL STRUCTURES: ASSESSMENT OF THE ACCURACY IN LIGAND-BOUND G PROTEIN-COUPLED RECEPTORS Article 06 December 2024

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Download references ACKNOWLEDGEMENTS We acknowledge A. Tsolakou, D. Milic and K.S. Harpsøe for help with data annotation; I. Carson for development of the preliminary version of the

Stabilising Mutation Analyser; and C.-J. Tsai for input on the description of cryo-EM construct engineering and experiments. This work was supported in part by the ERC (Starting Grant 639125

to D.E.G.), the Lundbeck Foundation (grants R163-2013-16327 and R218-2016-1266 to D.E.G.), the Swiss National Science Foundation (grant CRSII2_160805 to X.D.), the European Commisions

Seventh Framework Program (FP7/2007-2013; grant 290605 (COFUND: PSI-FELLOW) to E.M.) and the COST Action CM1207 (‘GLISTEN’). AUTHOR INFORMATION Author notes * Vignir Isberg Present address:

Novozymes A/S, Copenhagen, Denmark * These authors contributed equally: Christian Munk, Eshita Mutt. AUTHORS AND AFFILIATIONS * Department of Drug Design and Pharmacology, University of

Copenhagen, Copenhagen, Denmark Christian Munk, Vignir Isberg, Louise F. Nikolajsen, Janne M. Bibbe & David E. Gloriam * Paul Scherrer Institute, Villigen, Switzerland Eshita Mutt, 

Tilman Flock & Xavier Deupi * GPCR Consortium, San Marcos, CA, USA Michael A. Hanson * Departments of Biological Sciences and Chemistry, Bridge Institute, USC Michelson Center for

Convergent Bioscience, University of Southern California, Los Angeles, CA, USA Raymond C. Stevens * iHuman Institute, ShanghaiTech University, Shanghai, China Raymond C. Stevens Authors *

Christian Munk View author publications You can also search for this author inPubMed Google Scholar * Eshita Mutt View author publications You can also search for this author inPubMed Google

Scholar * Vignir Isberg View author publications You can also search for this author inPubMed Google Scholar * Louise F. Nikolajsen View author publications You can also search for this

author inPubMed Google Scholar * Janne M. Bibbe View author publications You can also search for this author inPubMed Google Scholar * Tilman Flock View author publications You can also

search for this author inPubMed Google Scholar * Michael A. Hanson View author publications You can also search for this author inPubMed Google Scholar * Raymond C. Stevens View author

publications You can also search for this author inPubMed Google Scholar * Xavier Deupi View author publications You can also search for this author inPubMed Google Scholar * David E.

Gloriam View author publications You can also search for this author inPubMed Google Scholar CONTRIBUTIONS C.M., D.E.G., J.M.B. and T.F. made the construct analyses and figures; C.M. and

V.I. developed the online resources; E.M. and C.M. annotated and analyzed published experimental data; L.F.N. conducted the mutagenesis experiments; M.A.H. and R.C.S. provided critical input

on the project, manuscript writing and data analysis; X.D. and D.E.G. drafted the paper; all authors commented on the drafted manuscript; D.E.G. and X.D. designed the project; and D.E.G.

managed the project. CORRESPONDING AUTHORS Correspondence to Christian Munk or David E. Gloriam. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing interests.

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SUPPLEMENTARY TEXT AND FIGURES Supplementary Note 1 and Supplementary Tables 1–5 RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Munk, C., Mutt, E.,

Isberg, V. _et al._ An online resource for GPCR structure determination and analysis. _Nat Methods_ 16, 151–162 (2019). https://doi.org/10.1038/s41592-018-0302-x Download citation *

Received: 18 April 2018 * Accepted: 14 December 2018 * Published: 21 January 2019 * Issue Date: February 2019 * DOI: https://doi.org/10.1038/s41592-018-0302-x SHARE THIS ARTICLE Anyone you

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