Real-time mirroring of therapy


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Access through your institution Buy or subscribe Early assessment of treatment efficacy can be invaluable in cases of aggressive tumours, such as glioblastoma. Logun et al. generated


glioblastoma organoids (GBOs) from patients participating in a chimeric antigen receptor (CAR) T cell clinical trial to monitor the therapeutic response in real time. The authors obtained


tumour specimens from six patients with glioblastoma enrolled in a phase 1 CAR T cell clinical trial. Using a previously reported protocol, they developed GBOs from the specimens in 2–3


weeks, which they then co-cultured with patient-matched CAR T cells. These GBOs displayed tumour cytolysis. After 6 days of co-culturing, Logun et al. collected the GBOs and analysed them


for target antigen expression, which they found was reduced. Finally, they analysed CAR T cell–GBO co-culture media and found a continuous release of cytokines by the activated T cells,


which presented similar temporal dynamics with the in vivo kinetics of CAR T cell activation in patients. This is a preview of subscription content, access via your institution ACCESS


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FAQs * Contact customer support AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Nature Cell Biology https://www.nature.com/ncb/ Stylianos Lefkopoulos Authors * Stylianos Lefkopoulos View


author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Stylianos Lefkopoulos. RIGHTS AND PERMISSIONS Reprints and permissions


ABOUT THIS ARTICLE CITE THIS ARTICLE Lefkopoulos, S. Real-time mirroring of therapy. _Nat Cell Biol_ 27, 4 (2025). https://doi.org/10.1038/s41556-024-01600-x Download citation * Published:


16 January 2025 * Issue Date: January 2025 * DOI: https://doi.org/10.1038/s41556-024-01600-x SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:


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