Plasma membrane lipid diffusion

Access through your institution Buy or subscribe Signal transduction from the plasma membrane involves local recruitment and activation of proteins, which can occur via the lipid PI(4,5)P2

(phosphatidyl-D-_myo_-inositol-4,5-bisphosphate). Whether its binding partners indirectly enrich the lipid at plasma membrane subdomains or pools of the lipids are first created locally has

been unclear. Pacheco et al. have now determined the trajectories of PI(4,5)P2 at the plasma membrane and how they may be affected by PI(4,5)P2-binding complexes. Using genetically encoded

lipid biosensors and single-particle tracking photoactivation localization microscopy, the researchers monitored the diffusion of unbound lipids, with a resolution of ∼100 nm and ∼100 ms.

They describe two modes of diffusion: a largely Brownian diffusion mode; and a slower, more anomalous pattern. They analyzed HeLa cells expressing fluorescence-labelled proteins associated

with PI(4,5)P2; surprisingly, PI(4,5)P2 diffused freely in the membrane regardless of clathrin-containing structures, accumulation of extended synaptotagmin 1, or cortical actin. However,

the mobility of PI(4,5)P2 was affected by septin or spectrin filaments, which are anchored in the membrane in a PI(4,5)P2-dependent manner. This is a preview of subscription content, access

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institutional subscriptions * Read our FAQs * Contact customer support AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Nature Cell Biology https://www.nature.com/ncb Melina Casadio Authors *

Melina Casadio View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Melina Casadio. RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Casadio, M. Plasma membrane lipid diffusion. _Nat Cell Biol_ 25, 6 (2023). https://doi.org/10.1038/s41556-022-01076-7 Download citation *

Published: 17 January 2023 * Issue Date: January 2023 * DOI: https://doi.org/10.1038/s41556-022-01076-7 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this

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