Surfactant influences signalling functions of the adhesion molecule l-selectin


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Abstract 92 BACKGROUND: Leukocyte trafficking to inflammatory sites is initiated by leukocyte rolling mediated by the selectin family of adhesion molecules. Adhesion of leukocytes to


endothelial cells and extravasation in the intraalveolar space is thought to be critical for induction of acute lung injury. Recently, we identified several intracellular signalling cascades


upon L-selectin triggering in leukocytes, resulting in synthesis of oxygen radicals, actin polymerization, activation of the neutral sphingomyelinase and the Ras/MAP-kinase pathway. Aim of


the present study is to examine potential effects of surfactant on L-selectin induced signalling. METHODS: Immune fluorescence, kinase/sphingomyelinase assays, flow cytometry in L-selectin


stimulated T-lymphocytes preincubated with surfactant. RESULTS: Treatment of lymphocytes with different surfactant preparations (Exosurf™, Curosurf™) blocks L-selectin induced lymphocyte


activation. In particular, surfactant prevented capping of the L-selectin receptor molecule and the activation of the src-like tyrosine kinase p56lck after L-selectin triggering.


Furthermore, the neutral sphingomyelinase, which is also activated by L-selectin, is inhibited by surfactant already in resting cells. Surfactant did not influence receptor binding by


antibodies as shown in FACS-analysis. CONCLUSIONS: Surfactant inhibits at least two independent signalling pathways triggered by L-selectin stimulation. The attenuation of L-selectin


signalling might result in modulation of leukocyte function and may contribute to the anti-inflammatory effects of surfactant. _Supported by a grant from the University Heidelberg_ AUTHOR


INFORMATION AUTHORS AND AFFILIATIONS * Department of Neonatology, Children's Hospital, INF 150, Heidelberg Birgit Brenner, Sonja Junge & Otwin Linderkamp Authors * Birgit Brenner


View author publications You can also search for this author inPubMed Google Scholar * Sonja Junge View author publications You can also search for this author inPubMed Google Scholar *


Otwin Linderkamp View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE


Brenner, B., Junge, S. & Linderkamp, O. Surfactant influences Signalling Functions of the Adhesion Molecule L-Selectin. _Pediatr Res_ 44, 434 (1998).


https://doi.org/10.1203/00006450-199809000-00125 Download citation * Issue Date: 01 September 1998 * DOI: https://doi.org/10.1203/00006450-199809000-00125 SHARE THIS ARTICLE Anyone you share


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