The distribution of granulocyte colony-stimulating factor receptor (g-csf-r) and its messenger rna expression in the human fetus ♦ 1380

Granulocyte colony-stimulating factor(G-CSF) is a lineage specific hematopoietic growth factor and a physiologic regulator of neutrophil proliferation, differentiation, function, and

survival. Its receptor, G-CSF-R, has been identified on the surface of nonhematopoietic cells including vascular endothelial cells, human placental trophoblasts, and cell lines derived from

small cell carcinoma of the lung. G-CSF affects the growth and activity of each of these various cell types. Responsiveness to G-CSF by nonhematopoietic cells may have important clinical

consequences if recombinant G-CSF is used therapeutically. This may be especially true in the developing fetus and neonate. Therefore, we hypothesized that G-CSF-R would be present in

extrahematopoietic fetal tissue and sought to evaluate the anatomical distribution of the receptor using immunohistochemical and messenger RNA determinations. Fetal tissues including brain,

spinal cord, lung, heart, liver, kidney, adrenal, intestine, muscle, and vasculature were isolated from abortuses at 11, 18, and 24 wks gestation (N=5). Tissue for immunohistochemical

staining was preserved in Bouin's fixative and transferred to 80% ethanol. Tissue for mRNA determination was washed extensively and immediately frozen at -80°C until RT-PCR analysis. We

found that the G-CSF-R was widely expressed anatomically in these fetuses as determined by both immunohistochemical staining and mRNA analysis. Immunoreactivity for G-CSF-R was observed in

vascular endothelial cells of major vessels in each organ, macrophages within the liver parenchyma, the collecting tubules of the kidney, mucosal and submucosal cells of intestinal villi,

chromaffin cells of the adrenal glands, terminal bronchioles of the developing respiratory tract, and within the retina of the eye. RT-PCR demonstrated the expression of the G-CSF-R in every

tissue examined. The presence of G-CSF-R on these nonhematopoietic tissues and the potential for responsiveness to G-CSF may have important implications when recombinant G-CSF is

administered to neonates. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Pediatrics, University of Florida, Gainesville, FL, USA Darlene A Calhoun & Robert D Christensen Authors * Darlene

A Calhoun View author publications You can also search for this author inPubMed Google Scholar * Robert D Christensen View author publications You can also search for this author inPubMed 

Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Calhoun, D., Christensen, R. The Distribution of Granulocyte Colony-Stimulating Factor

Receptor (G-CSF-R) and Its Messenger RNA Expression in the Human Fetus ♦ 1380. _Pediatr Res_ 43 (Suppl 4), 236 (1998). https://doi.org/10.1203/00006450-199804001-01401 Download citation *

Issue Date: 01 April 1998 * DOI: https://doi.org/10.1203/00006450-199804001-01401 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

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