Biochemical manifestations of oxygen toxicity in the newborn lamb

ABSTRACT ABSTRACT: The purpose of this project was to study the role of lipid peroxidation in oxygen-induced lung injury in the newborn lamb. It was our hypothesis that injury to the

microvascular bed of the lung by oxygen would coincide with a burst of peroxidative activity and would be accompanied by an increased rate of excretion of ethane and pentane in expired gas.

We measured vascular pressures, the rate of lung lymph flow and concentrations of ethane and pentane in exhaled gas in 10 newborn lambs that breathed >95% oxygen continuously. Our marker

for oxygen-induced lung injury was an increase in the permeability of the microvascular bed of the lung to protein (an increase in the rate of lung lymph flow accompanied by an increase in

the protein concentration in lymph). Although all 10 lambs demonstrated an abrupt increase in microvascular permeability to protein within 48 to 96 h of exposure to >95% oxygen, the rates

of ethane and pentane excretion remained unchanged throughout the entire experimental period. Lung tissue concentrations of glutathione decreased by 40% in the oxygen-exposed lambs and the

concentrations of glutathione disulfide increased 85% relative to air-breathing controls. Activities of glutathione reductase and superoxide dismutase were lower in the lungs of the oxygen-

exposed lambs than in controls, whereas the activities of glutathione peroxidase and catalase were not changed. We conclude that, in the lamb, changes in the rates of excretion of ethane and

pentane do not correlate with the timing of injury to the microvascular bed of the lung. SIMILAR CONTENT BEING VIEWED BY OTHERS EXPOSURE TO HIGH LEVELS OF OXYGEN IN NEONATAL RATS INDUCE A

DECREASE IN HEMOGLOBIN LEVELS Article 30 October 2021 SHORT EXPOSURE TO HYPEROXIA CAUSES CULTURED LUNG EPITHELIAL CELL MITOCHONDRIAL DYSREGULATION AND ALVEOLAR SIMPLIFICATION IN MICE Article

03 November 2020 POSTNATAL HYPOXIC PRECONDITIONING ATTENUATES LUNG DAMAGE FROM HYPEROXIA IN NEWBORN MICE Article 24 September 2024 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Department of Pediatrics, Section of Neonatology, Baylor College of Medicine, Houston, 77030, Texas Thomas N Hansen, Charles V Smith, Alfred L Gest, Harilyn W Smith & Mark Giesler

Authors * Thomas N Hansen View author publications You can also search for this author inPubMed Google Scholar * Charles V Smith View author publications You can also search for this author

inPubMed Google Scholar * Alfred L Gest View author publications You can also search for this author inPubMed Google Scholar * Harilyn W Smith View author publications You can also search

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ABOUT THIS ARTICLE CITE THIS ARTICLE Hansen, T., Smith, C., Gest, A. _et al._ Biochemical Manifestations of Oxygen Toxicity in the Newborn Lamb. _Pediatr Res_ 28, 613–617 (1990).

https://doi.org/10.1203/00006450-199012000-00014 Download citation * Received: 26 January 1990 * Accepted: 08 August 1990 * Issue Date: 01 December 1990 * DOI:

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