Anion transport in sickle red blood cells

ABSTRACT ABSTRACT: Anion transport in sickle cells (SS RBC) mediated by the band 3 membrane protein was evaluated by three different measures in both oxygenated and deoxygenated conditions

and compared to normal red cells. First, Cl- self-exchange measured as 36Cl- efflux at 0°C was normal in SS RBC in both Vmax and dependence on extracellular Cl- concentration. There was no

effect of deoxygenation on either parameter. Second, stilbene-sensitive 35SO=4:SO=4exchange, measured at 37°C where morphologic sickling occurred, was also unaffected by deoxygenation and

was normal compared to normal red cells. Third, conductive Cl- flux was assessed by measuring the rates of Cl−-limited K+ efflux in valinomycin-treated cells at 37°C. Both the

stilbene-sensitive and insensitive components of net Cl- flux were similar in SS RBC and normal red cells, and were unaltered by morphologic sickling. Thus, despite dramatic alterations in

cation transport in SS RBC and the demonstration of interaction between band 3 protein and sickle cell, anion transport functions appear to be normal in SS RBC and are unaffected by

deoxygenation. These data suggest that the majority of the anion exchangers in SS RBC are functionally normal. SIMILAR CONTENT BEING VIEWED BY OTHERS CYTOPLASMIC ANION/CATION IMBALANCES

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Article Open access 18 November 2020 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departments of Pediatrics and Physiology and Biophysics, and Perinatal Research Institute,

University Cincinnati College of Medicine, Cincinnati, 45267-0541, Ohio Clinton H Joiner * Department of Physiology and Biophysics, Emory University, Atlanta, 30322, Georgia Robert B Gunn 

& Otto Fröhlich * Department of Pediatrics, University of Alabama at Birmingham, Birmingham, 35294, Alabama Clinton H Joiner Authors * Clinton H Joiner View author publications You can

also search for this author inPubMed Google Scholar * Robert B Gunn View author publications You can also search for this author inPubMed Google Scholar * Otto Fröhlich View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Joiner, C., Gunn, R. &

Fröhlich, O. Anion Transport in Sickle Red Blood Cells. _Pediatr Res_ 28, 587–590 (1990). https://doi.org/10.1203/00006450-199012000-00008 Download citation * Received: 09 April 1990 *

Accepted: 08 August 1990 * Issue Date: 01 December 1990 * DOI: https://doi.org/10.1203/00006450-199012000-00008 SHARE THIS ARTICLE Anyone you share the following link with will be able to

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