Biochemical properties of tracheobronchial mucins from cystic fibrosis and non-cystic fibrosis individuals

ABSTRACT ABSTRACT. Tracheobronchial mucins from healthy individuals and from patients with bronchial asthma or cystic fibrosis (CF) were isolated from lung mucus, purified, and their

chemical and physical properties compared. Normal and asthmatic mucins required both a dissociating and a reducing agent for solubilization and exhibited identical chromatographic behavior

on Sepharose 4B, Sepharose 2B, and hydroxylapatite and similar amino acid and carbohydrate compositions. In contrast, 1) CF lung mucins were solubilized in the absence of dissociating and/or

reducing agents and 2) the majority of the CF mucins analyzed was eluted in the included volume of Sepharose 4B with Kd values of 0.3 ± 0.1 rather than in the void volume and thus appeared

smaller than normal and asthmatic mucins. The lower molecular weight mucins in CF sputum apparently are produced by bacterial or inflammatory cell proteinases since radiolabelled asthmatic

mucin was digested to smaller fragments when incubated with crude CF lung mucosal samples. Furthermore, mucins secreted by tracheal explants from CF and from non-CF individuals eluted in the

void volume on Sepharose 4B, suggesting that CF tracheobronchial mucins were not inherently smaller than non-CF mucins. SIMILAR CONTENT BEING VIEWED BY OTHERS RHEOLOGICAL COMPARISON OF

SPUTUM AND RECONSTITUTED AIRWAY EPITHELIUM MUCUS Article Open access 30 December 2024 CFTR EXPRESSION DECREASES WITH AGE IN SEVERAL AIRWAY CELL TYPES Article Open access 21 November 2024

REDUCED SIALYLATION OF AIRWAY MUCIN IMPAIRS MUCUS TRANSPORT BY ALTERING THE BIOPHYSICAL PROPERTIES OF MUCIN Article Open access 17 July 2024 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Department of Child Health and Development, George Washington University, Children's Hospital National Medical Center, Washington, 20010, DC. Mary Callaghan Rose & 

John Z Jacoby III * Department of Biochemistry, Duke University Medical Center, Durham, 27705, North Carolina Charles F Brown, W S Lynn & Bernard Kaufman Authors * Mary Callaghan Rose

View author publications You can also search for this author inPubMed Google Scholar * Charles F Brown View author publications You can also search for this author inPubMed Google Scholar *

John Z Jacoby III View author publications You can also search for this author inPubMed Google Scholar * W S Lynn View author publications You can also search for this author inPubMed Google

Scholar * Bernard Kaufman View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS

ARTICLE Callaghan Rose, M., Brown, C., Jacoby, J. _et al._ Biochemical Properties of Tracheobronchial Mucins from Cystic Fibrosis and Non-Cystic Fibrosis Individuals. _Pediatr Res_ 22,

545–551 (1987). https://doi.org/10.1203/00006450-198711000-00015 Download citation * Received: 24 November 1968 * Accepted: 26 June 1987 * Issue Date: 01 November 1987 * DOI:

https://doi.org/10.1203/00006450-198711000-00015 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is

not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative