Studies on the intestinal surface acid microclimate: developmental aspects


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ABSTRACT ABSTRACT. The existence and general characteristics of the intestinal surface acid microclimate (ISAM) in the developing intestine of suckling and weanling rats were examined. ISAM


pH measurements were performed _in vitro_ using a sensitive glass pH-microelectrode. The results showed that the ISAM does exist in both suckling and weanling rat intestine. In both suckling


and weanling rats, ISAM pH was significantly (_p_ < 0.01) lower in the jejunum than in the ileum, an observation similar to that previously reported in the small intestine of adult rats.


In the colon, however, ISAM pH of suckling rats was significantly (_p_ < 0.01) lower than that of weanling and adult rats. Studies on the relationship between jejunal ISAM pH of weanling


rats and incubation buffer pH showed that the two are not in equilibrium. Jejunal ISAM pH of weanling rats was significantly inhibited by: 1) the mucolytic agent N-acetyl- L-cysteine, 2)


stirring of the incubation medium, 3) Na+ removal, 4) glucose removal (or substitution by the unmetabolizable galactose), and 5) metabolic inhibitors (iodoacetate and dinitrophenol). These


results demonstrate the existence of the ISAM in the developing intestine of suckling and weanling rats and shows the dependence of the ISAM on Na+, metabolizable substrate(s) and normal


intracellular metabolism. Furthermore, surface mucus appears to play a role in maintaining the ISAM, most probably through retaining the H+ at the intestinal surface. SIMILAR CONTENT BEING


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— THE SMALL INTESTINAL MICROBIOTA Article 23 October 2024 ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Departments of Pediatric Gastroenterology, Molecular Physiology and


Biophysics, Vanderbilt University School of Medicine, Nashville, 37232, Tennessee Hamid M Said, Rita Smith & Reyadh Redha Authors * Hamid M Said View author publications You can also


search for this author inPubMed Google Scholar * Rita Smith View author publications You can also search for this author inPubMed Google Scholar * Reyadh Redha View author publications You


can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Said, H., Smith, R. & Redha, R. Studies on


the Intestinal Surface Acid Microclimate: Developmental Aspects. _Pediatr Res_ 22, 497–499 (1987). https://doi.org/10.1203/00006450-198711000-00002 Download citation * Received: 23 February


1987 * Accepted: 29 May 1987 * Issue Date: 01 November 1987 * DOI: https://doi.org/10.1203/00006450-198711000-00002 SHARE THIS ARTICLE Anyone you share the following link with will be able


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