260 dissociation of thyroid hormone stimulated respira-tion and na/k atpase activity in brown adipose tissue (bat) and liver in the newborn rabbit


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ABSTRACT The effects of triiodothyronine (T3) on oxygen consumption (QO2), Na/K ATPase activity and mitochondrial alpha glycerophosphate dehydrogenase (αGPD) activity in BAT and liver were


studied in newborn rabbits. Each of seven 5 day old litters was divided in half, and the newborns injected with either T3 (20 μg/100 g BW/day, SC) or vehicle for 3 days. Basal QO2 with and


without ouabain was determined on isolated BAT cells or chopped liver. αGPD and Na/K ATPase were measured on tissue from individual animals. T3 stimulated BAT QO2, mean (±SEM)=119±18 vs 65±4


μl O2/106 cells ·hr in controls, p<0.005; there was no effect of ouabain in either group. BAT αGPD increased in response to T3 (1850±119 ΔOD × 103/mg protein·min vs 1266±121 in controls,


p<0.005), but Na/K ATPase was unchanged. Hepatic QO2 was similar in T3 treated and control animals; ouabain suppressed respiration by ∼30% in both groups. Hepatic αGPD showed no response


to T3, while Na/K ATPase was significantly increased (0.307±.025 μMPi/mg protein·hr vs 0.202±.021 in controls, p<0.003). Conclusions: 1) BAT respiration and mitochondrial αGPD but not


Na/K ATPase are thyroid sensitive in the newborn rabbit. 2) Hepatic Na/K -ATPase activity is responsive to T3 but αGPD and respiration are not. 3) T3 stimulation of respiration is


dissociated from changes in Na/K ATPase activity in liver and BAT. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Pediatrics, UCLA School of Medicine, Harbor-UCLA


Medical Center, Torrance Alan H Klein, Jennifer Jenkins, Anita Reviczky & Delbert A Fisher Authors * Alan H Klein View author publications You can also search for this author inPubMed 


Google Scholar * Jennifer Jenkins View author publications You can also search for this author inPubMed Google Scholar * Anita Reviczky View author publications You can also search for this


author inPubMed Google Scholar * Delbert A Fisher View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT


THIS ARTICLE CITE THIS ARTICLE Klein, A., Jenkins, J., Reviczky, A. _et al._ 260 DISSOCIATION OF THYROID HORMONE STIMULATED RESPIRA-TION AND Na/K ATPase ACTIVITY IN BROWN ADIPOSE TISSUE


(BAT) AND LIVER IN THE NEWBORN RABBIT. _Pediatr Res_ 15 (Suppl 4), 483 (1981). https://doi.org/10.1203/00006450-198104001-00271 Download citation * Issue Date: 01 April 1981 * DOI:


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