1024 monoclonal antibodies protect against fatal group b streptococcal (gbs) infection in mice


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ABSTRACT Despite the use of broad spectrum antimicrobial agents, GBS sepsis remains a fulminant, often fatal disease. Using the technique of somatic cell hybridization, we have developed


hybridomas which secrete monoclonal antibodies that react with each of the 5 serotypes of GBS. The purpose of this investigation was: 1) to determine whether type-specific monoclonal


antibody conferred protection against fatal GBS infection in mice, and 2) to study the functional properties of the antibodies (agglutination, complement fixation). BALB/c mice were given a


subscapular injection of 5 × 106 hybridoma cells that produced antibody with one of the following specificities: anti-GBS IA/IC, IB, or II. (Type III GBS does not infect adult BALB/c mice).


After 14 days, tumor mice and an equal number of controls were challenged with an I.P. injection of 109 live GBS of identical specificity to the tumor. 100% of mice with tumors survived;


controls were dead within 24 hours. Surviving tumor mice had anti-GBS titers of > 1/10,000 against the respective bacterial serotypes. Each monoclonal antibody fixed complement and


agglutinated bacteria of the same specificity. GBS monoclonal antibodies protect mice against fatal infection in vivo, fix complement and agglutinate GBS in vitro and may have potential use


in the therapy of life-threatening disease in infants. ARTICLE PDF AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * (Spon by W.W. Fox). Div. of Neonatology and Allergy-Immunology,


Children's Hospital of Philadelphia, and Univ. of Pa. Sch. of Med., Philadelphia, PA. Mary Catherine Harris, Judith A Bailey, Linda L Wright, Steven P Douglas & Richard A Polin


Authors * Mary Catherine Harris View author publications You can also search for this author inPubMed Google Scholar * Judith A Bailey View author publications You can also search for this


author inPubMed Google Scholar * Linda L Wright View author publications You can also search for this author inPubMed Google Scholar * Steven P Douglas View author publications You can also


search for this author inPubMed Google Scholar * Richard A Polin View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and


permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Harris, M., Bailey, J., Wright, L. _et al._ 1024 MONOCLONAL ANTIBODIES PROTECT AGAINST FATAL GROUP B STREPTOCOCCAL (GBS) INFECTION IN MICE.


_Pediatr Res_ 15 (Suppl 4), 613 (1981). https://doi.org/10.1203/00006450-198104001-01050 Download citation * Issue Date: 01 April 1981 * DOI: https://doi.org/10.1203/00006450-198104001-01050


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