Overexpression of mir-370 and downregulation of its novel target tgfβ-rii contribute to the progression of gastric carcinoma

ABSTRACT MicroRNAs (miRNAs) are endogenous non-coding RNAs that are known to be involved in the pathogenesis of tumors. Gastric carcinoma (GC) is a common malignancy worldwide. The aim of

this study was the identification of the expression signature and functional roles of aberrant miRNAs in GC. Initial screening established a profile of aberrantly expressed miRNAs in tumors.

_miR-370_ was confirmed to be overexpressed in GC tissues. Higher expression of _miR-370_ in GC tissues was associated with more advanced nodal metastasis and a higher clinical stage

compared with controls. In addition, significantly higher level of _miR-370_ was noted in the plasma of GC patients compared with controls. Patients having more invasive or advanced tumors

also exhibited a higher plasma level of _miR-370_. _In vitro_ assays indicated that exogenous _miR-370_ expression enhanced the oncogenic potential of GC cells. The AGS-GFPM2 cells with

exogenous _miR-370_ expression also exhibited enhanced abdominal metastatic dissemination in nude mice. Reporter assays confirmed that _miR-370_ targeted predicted sites in 3′UTR of

_transforming growth factor-β receptor II_ (_TGFβ-RII_) gene. The exogenous _miR-370_ expression decreased _TGFβ-RII_ expression and the phosphorylation of Smad3 elicited by TGFβ1. The

TGFβ1-mediated repression in cell migration was reverted by exogenous _miR-370_ expression. A reverse correlation between _miR-370_ and TGFβ-RII expression was noted in GC tissues. This

study concludes that _miR-370_ is a miRNA that is associated with GC progression by downregulating _TGFβ-RII_. The miRNA expression profile described in this study should contribute to

future studies on the role of miRNAs in GC. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS Access

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subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS MICRORNAS ARE INVOLVED IN THE DEVELOPMENT AND PROGRESSION OF GASTRIC CANCER Article 09 October

2020 THE MIR-532-E2F1 FEEDBACK LOOP CONTRIBUTES TO GASTRIC CANCER PROGRESSION Article Open access 19 April 2022 NOVEL PROGNOSTIC MARKER LINC00205 PROMOTES TUMORIGENESIS AND METASTASIS BY

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  Download references ACKNOWLEDGEMENTS We acknowledge helps from Dr Shu-Chun Lin, Dr Kuo-Wei Chang, Ms Yu-Pin Chen, Mr Shi-Wen Chen and Mr Chung-Sheng Chou. This study was supported by grant

96-2314-B-010-055-MY3 from National Science Council, and National Yang-Ming University Hospital Grant RD 2008-001. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Medicine,

School of Medicine, National Yang-Ming University, Taipei, Taiwan S-S Lo, W-L Fang, C-Y Chen, W-T Chen & C-W Wu * Department of Surgery, National Yang-Ming University Hospital, Yi-Lan,

Taiwan S-S Lo, C-Y Chen & W-T Chen * Department of Dentistry, National Yang-Ming University Hospital, Yi-Lan, Taiwan P-S Hung, H-F Tu & N-R Gong * Division of General Surgery,

Department of Surgery, Taipei Veterans General Hospital, Taipei, Taiwan J-H Chen, W-L Fang & C-W Wu Authors * S-S Lo View author publications You can also search for this author inPubMed

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THIS ARTICLE CITE THIS ARTICLE Lo, SS., Hung, PS., Chen, JH. _et al._ Overexpression of _miR-370_ and downregulation of its novel target TGFβ-RII contribute to the progression of gastric

carcinoma. _Oncogene_ 31, 226–237 (2012). https://doi.org/10.1038/onc.2011.226 Download citation * Received: 04 October 2010 * Revised: 29 April 2011 * Accepted: 09 May 2011 * Published: 13

June 2011 * Issue Date: 12 January 2012 * DOI: https://doi.org/10.1038/onc.2011.226 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

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* microRNA * _miR-370_ * plasma * stomach * TGFβ-RII