Chk1 c-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of chk1 catalytic activity

ABSTRACT Chk1 is phosphorylated within its C-terminal regulatory domain by the upstream ATM/ATR kinases during checkpoint activation; however, how this modulates Chk1 function is poorly

understood. Here, we show that Chk1 kinase activity is rapidly stimulated in a cell-cycle phase-specific manner in response to both DNA damage and replication arrest, and that the extent and

duration of activation correlates closely with regulatory phosphorylation at serines (S) S317, S345 and S366. Despite their evident co-regulation, substitutions of individual Chk1

regulatory sites with alanine (A) residues have differential effects on checkpoint proficiency and kinase activation. Thus, whereas Chk1 S345 is essential for all functions tested, mutants

lacking S317 or S366 retain partial proficiency for G2/M and S/M checkpoint arrests triggered by DNA damage or replication arrest. These phenotypes reflect defects in Chk1 kinase induction,

as the mutants are either partially (317A and 366A) or completely (345A) resistant to kinase activation. Importantly, S345 phosphorylation is impaired in Chk1 S317A and S366A mutants,

suggesting that modification of adjacent SQ sites promotes this key regulatory event. Finally, we provide biochemical evidence that Chk1 catalytic activity is stimulated by a de-repression

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Download references ACKNOWLEDGEMENTS We thank Dr Frank Kozielski for his comments on the manuscript. This work was supported by Cancer Research UK (CR-UK). AUTHOR INFORMATION AUTHORS AND

AFFILIATIONS * Beatson Institute for Cancer Research, Garscube Estate, Glasgow, UK M Walker, E J Black, V Oehler, D A Gillespie & M T Scott * Faculty of Biomedical and Life Sciences,

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ARTICLE Walker, M., Black, E., Oehler, V. _et al._ Chk1 C-terminal regulatory phosphorylation mediates checkpoint activation by de-repression of Chk1 catalytic activity. _Oncogene_ 28,

2314–2323 (2009). https://doi.org/10.1038/onc.2009.102 Download citation * Received: 27 February 2009 * Revised: 23 March 2009 * Accepted: 24 March 2009 * Published: 04 May 2009 * Issue

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