The structural basis for specificity in human abo(h) blood group biosynthesis

ABSTRACT The human ABO(H) blood group antigens are produced by specific glycosyltransferase enzymes. An N-acetylgalactosaminyltransferase (GTA) uses a UDP-GalNAc donor to convert the

H-antigen acceptor to the A antigen, whereas a galactosyltransferase (GTB) uses a UDP-galactose donor to convert the H-antigen acceptor to the B antigen. GTA and GTB differ only in the

identity of four critical amino acid residues. Crystal structures at 1.8–1.32 Å resolution of the GTA and GTB enzymes both free and in complex with disaccharide H-antigen acceptor and UDP

reveal the basis for donor and acceptor specificity and show that only two of the critical amino acid residues are positioned to contact donor or acceptor substrates. Given the need for

stringent stereo- and regioselectivity in this biosynthesis, these structures further demonstrate that the ability of the two enzymes to distinguish between the A and B donors is largely

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RETAINING Β-KDO GLYCOSYLTRANSFERASE WBBB USES A DOUBLE-DISPLACEMENT MECHANISM WITH AN INTERMEDIATE ADDUCT REARRANGEMENT STEP Article Open access 21 October 2022 _AKKERMANSIA MUCINIPHILA_

EXOGLYCOSIDASES TARGET EXTENDED BLOOD GROUP ANTIGENS TO GENERATE ABO-UNIVERSAL BLOOD Article 29 April 2024 ACCESSION CODES ACCESSIONS PROTEIN DATA BANK * 1LZ0 * 1LZ7 * 1LZI * 1LZJ REFERENCES

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salary support and funding. M.M.P. thanks the Natural Sciences and Engineering Research Council of Canada for funding. The authors thank D.R. Bundle and O. Hindsgaul for helpful discussions,

as well as C. Weeks, J. Berendzen and R.W. Grosse-Kuntsleeve for help while at Brookhaven National Laboratories beamlines X4A, X8C and X12C. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS *

Department of Biochemistry, Microbiology and Immunology, University of Ottawa, Ottawa, K1H 8M5, Canada Sonia I. Patenaude, Nina O.L. Seto, Svetlana N. Borisova & Stephen V. Evans *

Institute for Biological Sciences, National Research Council of Canada, Ottawa, K1A 0R6, Canada Nina O.L. Seto * Department of Chemistry, University of Alberta, Edmonton, T6G 2G2, Canada

Adam Szpacenko, Sandra L. Marcus & Monica M. Palcic Authors * Sonia I. Patenaude View author publications You can also search for this author inPubMed Google Scholar * Nina O.L. Seto

View author publications You can also search for this author inPubMed Google Scholar * Svetlana N. Borisova View author publications You can also search for this author inPubMed Google

Scholar * Adam Szpacenko View author publications You can also search for this author inPubMed Google Scholar * Sandra L. Marcus View author publications You can also search for this author

inPubMed Google Scholar * Monica M. Palcic View author publications You can also search for this author inPubMed Google Scholar * Stephen V. Evans View author publications You can also

search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Stephen V. Evans. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial

interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Patenaude, S., Seto, N., Borisova, S. _et al._ The structural basis for specificity in human

ABO(H) blood group biosynthesis. _Nat Struct Mol Biol_ 9, 685–690 (2002). https://doi.org/10.1038/nsb832 Download citation * Received: 22 April 2002 * Accepted: 17 July 2002 * Published: 19

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