Solution structure of the dna-binding domain of mafg

ABSTRACT The Maf family proteins, which constitute a subgroup of basic region-leucine zipper (bZIP) proteins, function as transcriptional regulators of cellular differentiation. Together

with the basic region, the Maf extended homology region (EHR), conserved only within the Maf family, defines the DNA binding specific to Mafs. Here we present the first NMR-derived structure

of the DNA-binding domain (residues 1–76) of MafG, which contains the EHR and the basic region. The structure consists of three α-helices and resembles the fold of the DNA-binding domain of

Skn-1, a developmental transcription factor of _Caenorhabditis elegans_. The structural similarity between MafG and Skn-1 enables us to propose a possible mechanism by which Maf family

proteins recognize their consensus DNA sequences. Access through your institution Buy or subscribe This is a preview of subscription content, access via your institution ACCESS OPTIONS

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for useful discussion, K. Yap for providing a program to calculate interhelical angles and T. O'Connor for critical reading of the manuscript. This work was supported by grants from

JSPS and TARA (T.T.); the Ministry of Education, Science, Sports and Culture of Japan (H.M. and M.Y.); JSPS and CREST (M.Y.); and PROBRAIN (H.M.). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS

* Institute of Basic Medical Science, University of Tsukuba, Tsukuba, 305-8575, Ibaraki, Japan Hideki Kusunoki, Hozumi Motohashi, Fumiki Katsuoka & Masayuki Yamamoto * Center for

Tsukuba Advanced Research Alliance, University of Tsukuba, Tsukuba, 305-8577, Ibaraki, Japan Hideki Kusunoki, Hozumi Motohashi, Masayuki Yamamoto & Toshiyuki Tanaka * Institute of

Applied Biochemistry, University of Tsukuba, Tsukuba, 305-8572, Ibaraki, Japan Akio Morohashi & Toshiyuki Tanaka * Banyu Tsukuba Research Institute, Tsukuba, 300-2611, Ibaraki, Japan

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declare no competing financial interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kusunoki, H., Motohashi, H., Katsuoka, F. _et al._ Solution

structure of the DNA-binding domain of MafG. _Nat Struct Mol Biol_ 9, 252–256 (2002). https://doi.org/10.1038/nsb771 Download citation * Received: 02 October 2001 * Accepted: 24 January 2002

* Published: 04 March 2002 * Issue Date: 01 April 2002 * DOI: https://doi.org/10.1038/nsb771 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content:

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