Ferryl intermediates of catalase captured by time-resolved weissenberg crystallography and uv-vis spectroscopy

ABSTRACT Various enzymes use semi-stable ferryl intermediates and free radicals during their catalytic cycle, amongst them haem catalases. Structures for two transient intermediates

(compounds I and II) of the NADPH-dependent catalase from _Proteus mirabilis_ (PMC) have been determined by time-resolved X-ray crystallography and single crystal microspectrophotometry. The

results show the formation and transformation of the ferryl group in the haem, and the unexpected binding of an anion during this reaction at a site distant from the haem. Access through

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references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Laboratory of Molecular Biophysics and Oxford Centre for Molecular Sciences, University of Oxford, South Parks Road, Oxford, OX1 3QU,

UK Patrice Gouet, Pamela A. Williams & Janos Hajdu * Institut de Biologie Structural Jean-Pierre Ebel, Laboratoire d'Enzymologie Moléculaire(CNRS-CEA), 41 avenue des Martyrs,

38027, Grenoble Cedexl, France Hélène-Marie Jouve & Pierre Andreoletti * Department of Molecular Biology, Swedish University of Agricultural Sciences, Uppsala Biomedical Center, P.O. Box

590, S-75124, Uppsala, Sweden Inger Andersson * Département d'Ecophysiologie végétale et de microbiologie, Centre d'Etudes Nucleates de Cadarache, 13108, Saint-Paul lez Durance

Cedex, France Laurent Nussaume * Department of Biochemistry, Uppsala University, Box 576, S-751 23, Uppsala, Sweden Janos Hajdu Authors * Patrice Gouet View author publications You can also

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publications You can also search for this author inPubMed Google Scholar * Inger Andersson View author publications You can also search for this author inPubMed Google Scholar * Pierre

Andreoletti View author publications You can also search for this author inPubMed Google Scholar * Laurent Nussaume View author publications You can also search for this author inPubMed 

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ARTICLE Gouet, P., Jouve, HM., Williams, P. _et al._ Ferryl intermediates of catalase captured by time-resolved Weissenberg crystallography and UV-VIS spectroscopy. _Nat Struct Mol Biol_ 3,

951–956 (1996). https://doi.org/10.1038/nsb1196-951 Download citation * Received: 16 July 1996 * Accepted: 30 August 1996 * Issue Date: 01 November 1996 * DOI:

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