In Brief | Nature Reviews Microbiology

Access through your institution Buy or subscribe REAL-TIME PCR QUANTITATION OF _CLOSTRIDIA_ IN FECES OF AUTISTIC CHILDREN Song, Y. _et al_. _Appl. Environ. Microbiol._ 70, 6459–6465 (2004)

In the latest issue of _Applied and Environmental Microbiology_, Song _et al_., building on previous culture-based studies, describe the development of quantitative PCR assays that can be

used to quantify _Clostridia_ clusters in stool samples. The authors designed _Clostridia_ phylogenetic-cluster-specific oligonucleotide probes and PCR primers, and used stringent conditions

to avoid amplification of unwanted sequences from closely related species. The fluorescent signal from the PCR was correlated with cell density using a plate-count assay, for which careful

standard curves were selected. The assay is fast, sensitive and reliable, with >99% reproducibility. This is a preview of subscription content, access via your institution ACCESS OPTIONS

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(2005). https://doi.org/10.1038/nrmicro1083 Download citation * Issue Date: January 2005 * DOI: https://doi.org/10.1038/nrmicro1083 SHARE THIS ARTICLE Anyone you share the following link

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