Coordinated positioning | Nature Reviews Molecular Cell Biology

Access through your institution Buy or subscribe Factors that determine PCP are localized apically in fly imaginal-disc cells. Among them, Frizzled (Fz1) partially colocalizes with several

determinants of A–B polarity, including the PALS-1-associated tight junction protein (Patj)-containing complex and a complex that comprises Bazooka (Baz), Par6 and atypical protein kinase C

(aPKC). Marek Mlodzik and colleagues showed that one of these apical factors, aPKC, phosphorylates the C terminus of Fz1. Overexpression of wild-type Fz1 or a phosphorylation-defective

mutant caused a gain-of-function PCP phenotype, whereas a mutant that mimics phosphorylated Fz1 had a reduced gain-of-function phenotype. So, aPKC phosphorylation seems to inhibit Fz1

activity. Next, the authors asked how aPKC is recruited to the C terminus of Fz1. As it turned out, it was through another apical determinant of A–B polarity, Patj. Overexpression of a Fz1

mutant that lacked the Patj-binding site caused a stronger gain-of-function PCP phenotype compared with overexpression of wild-type Fz1, which indicates that aPKC and Patj are required for

the inhibition of Fz1-mediated PCP. This is a preview of subscription content, access via your institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12

print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be

subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support ORIGINAL

RESEARCH PAPER * Djiane, A. et al. The apical determinants aPKC and dPatj regulate Frizzled-dependent planar cell polarity in the Drosophila eye. _Cell_ 121, 621–631 (2005) Article  CAS 

Google Scholar  Download references Authors * Arianne Heinrichs View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and

permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Heinrichs, A. Coordinated positioning. _Nat Rev Mol Cell Biol_ 6, 513 (2005). https://doi.org/10.1038/nrm1686 Download citation * Published:

15 June 2005 * Issue Date: 01 July 2005 * DOI: https://doi.org/10.1038/nrm1686 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable

link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the Springer Nature SharedIt content-sharing initiative