Polarity and crtam: a matter of timing

Access through your institution Buy or subscribe CRTAM is an immunoglobulin-superfamily transmembrane protein that contains a PDZ domain, indicating that it might be capable of assembling

protein complexes at the cell membrane. Here, the authors showed that CRTAM is transiently expressed, between 6 and 24 hours following T-cell receptor (TCR) ligation, on the surface of

∼10–40% of naive CD4+ T cells. Compared with wild-type cells, CRTAM-deficient CD4+ T cells produced lower levels of IFNγ and IL-22 (and to a lesser extent IL-17), but similar levels of IL-4,

and had a higher rate of proliferation following activation. Within 5–30 minutes of TCR engagement, CD3, talin and other proteins such as protein kinase Cθ (PKCθ) are known to be recruited

to the TCR to form the immunological synapse. By studying later time points, Yeh _et al_. showed that co-localization of PKCθ with CD3 is no longer maintained. Instead, CRTAM, Scrib and PKCζ

co-localize with CD3 and talin at 8 and 12 hours following T-cell activation. Further analyses showed that CRTAM directly interacts with Scrib, which is known to be important in cell

polarity, and that a loss of CRTAM expression resulted in a loss of T-cell polarity in this later phase of T-cell activation. This is a preview of subscription content, access via your

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article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in

* Learn about institutional subscriptions * Read our FAQs * Contact customer support ORIGINAL RESEARCH PAPER * Yeh, J.-H., Sidhu, S. S. & Chan, A. C. Regulation of a late phase of T cell

polarity and effector functions by Crtam. _Cell_ 132, 846–859 (2008) Article  CAS  Google Scholar  Download references Authors * Olive Leavy View author publications You can also search for

this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Leavy, O. Polarity and CRTAM: a matter of timing. _Nat Rev Immunol_

8, 246 (2008). https://doi.org/10.1038/nri2308 Download citation * Issue Date: April 2008 * DOI: https://doi.org/10.1038/nri2308 SHARE THIS ARTICLE Anyone you share the following link with

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