Culturomics: illuminating microbial dark matter

Access through your institution Buy or subscribe 'Culturomics' uses multiple high-throughput culture conditions with mass spectroscopy or 16S ribosomal RNA sequencing for the

identification of previously unculturable bacterial species (often termed 'dark matter'). Now, the culturomics process has been further refined and used to double the number of

bacterial species isolated from the human gut. First, the investigators deduced the 18 best culture conditions and implemented new protocols (fresh-sample inoculation and microcolony

detection) with a focus on the culture of Proteobacteria and halophilic or microaerophilic bacteria. Using the refined conditions with a range of stool, small bowel and colonic samples from

healthy individuals and patients with various diseases from different geographical origins, 1,057 prokaryotic species were isolated, including 197 potentially new species. By genome

sequencing the new species and comparing the data with metagenomic studies from the Human Microbiome Project, the researchers confirmed that culturomics enables the culture of microorganisms

that correspond to previously unassigned sequences. This is a preview of subscription content, access via your institution RELEVANT ARTICLES Open Access articles citing this article. *

INTEGRATING THE MILK MICROBIOME SIGNATURES IN MASTITIS: MILK-OMICS AND FUNCTIONAL IMPLICATIONS * Rine Christopher Reuben *  & Carmen Torres _World Journal of Microbiology and

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ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Lagier, J.-C. _ et al_. Culture of previously uncultured

members of the human gut microbiota by culturomics. _Nat. Microbiol._ http://dx.doi.org/10.1038/nmicrobiol.2016.203 (2016) Download references Authors * Iain Dickson View author

publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Dickson, I. Culturomics:

illuminating microbial dark matter. _Nat Rev Gastroenterol Hepatol_ 14, 3 (2017). https://doi.org/10.1038/nrgastro.2016.189 Download citation * Published: 23 November 2016 * Issue Date:

January 2017 * DOI: https://doi.org/10.1038/nrgastro.2016.189 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get shareable link Sorry, a

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