Splicing steps aside to consider its options

Access through your institution Buy or subscribe Tyagi and colleagues applied fluorescent hybridization probes to track splicing events; the use of up to hundreds of fluorophores amplified

the signals and allowed the visualization and counting of single transcript molecules. The authors studied a _GFP_ transcript with two engineered introns — consisting of canonical splicing

signal sequences and distinct probe-binding sites — in Chinese hamster ovary cells. They probed the 3′ untranslated region (UTR) to localize all _GFP_ transcripts and monitored the

progression of splicing through the loss of colocalization with the intronic probes. They found that one of the introns was always spliced out co-transcriptionally but that splicing of the

other was uncoupled from transcription so that this intron was retained in the transcript throughout the nucleoplasm. This effect was independent of the order of the introns in the

construct, indicating that uncoupling from transcription can be an intrinsic feature of an intron. This is a preview of subscription content, access via your institution ACCESS OPTIONS

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institutional subscriptions * Read our FAQs * Contact customer support ORIGINAL RESEARCH PAPER * Vargas, D. Y. et al. Single-molecule imaging of transcriptionally coupled and uncoupled

splicing. _Cell_ 147, 1054–1065 (2011) Article Article  CAS  Google Scholar  Download references Authors * Darren J. Burgess View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Burgess, D. Splicing steps aside to consider its options. _Nat Rev Genet_ 13, 3

(2012). https://doi.org/10.1038/nrg3137 Download citation * Published: 06 December 2011 * Issue Date: January 2012 * DOI: https://doi.org/10.1038/nrg3137 SHARE THIS ARTICLE Anyone you share

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