Reprogramming metabolic flux in glioma

Access through your institution Buy or subscribe A new study has shown that growth of gliomas with isocitrate dehydrogenase 1 (_IDH1_) mutations is promoted by expression of glutamate

dehydrogenase 2 (GLUD2) protein. In order to determine promoters of growth in IDH1-mutated gliomas, Chen _et al_. established a glioma progenitor cell culture line using murine

nestin-positive, _Tp53_-negative neural stem cells. _IDH1_ mutations in human gliomas co-segregate with p53 mutations, thus these glioma progenitor cells are relevant to human tumours. The

authors examined the growth of glioma progenitor cells that also had either wild-type IDH1, shRNA-mediated knockdown of IDH1, or expression of the most common IDH1 mutant, IDH1-R132H.

Interestingly, cells lacking IDH1 or with IDH1-R132H grew more slowly, and, as expected, mutant IDH1 cells had elevated levels of 2HG. However, growth defects in IDH1-mutant cells could be

rescued with transfection of wild-type IDH1 in spite of enhanced accumulation of 2HG, suggesting that lack of αKG rather than the effects of 2HG is behind the growth defects in these cells.

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calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in * Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES * Chen, R. et al.

Hominoid-specific enzyme GLUD2 promotes growth of _IDH1__R132H_ glioma. _Proc. Natl Acad. Sci. USA_ http://dx.doi.org/10.1073/pnas.1409653111 (2014) Download references Authors * Isabel

Lokody View author publications You can also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Lokody, I.

Reprogramming metabolic flux in glioma. _Nat Rev Cancer_ 14, 706–707 (2014). https://doi.org/10.1038/nrc3840 Download citation * Published: 06 October 2014 * Issue Date: November 2014 * DOI:

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