Isolation of tcm cells for tumour immunotherapy

Access through your institution Buy or subscribe Animal studies have suggested that TCM cells can survive long-term after adoptive transfer. Human TCM cells have been reported to produce

more interleukin-2 (IL-2) and less interferon-γ (IFNγ) than effector memory T cells (TEM cells), and so the authors hypothesized that these cell populations could be identified on the basis

of their relative production of these cytokines. Indeed, a high _IL2_/_IFNG_ mRNA ratio in peripheral blood cells following short-term T cell stimulation correlated with the TCM cell

phenotype. Using a previously described high-throughput method to screen for tumour-specific CD8+ T cells, rare tumour-specific TCM cells were isolated from patients with melanoma on the

basis of their high _IL2_/_IFNG_ mRNA ratio. Effector T cell clones generated from tumour-specific TEM cells (which have a low _IL2_/_IFNG_ mRNA ratio) or from TCM cells had similar

cytolytic activities, but TCM cell-derived T cell clones had a distinct gene expression profile associated with cell survival. This is a preview of subscription content, access via your

institution ACCESS OPTIONS Access through your institution Subscribe to this journal Receive 12 print issues and online access $209.00 per year only $17.42 per issue Learn more Buy this

article * Purchase on SpringerLink * Instant access to full article PDF Buy now Prices may be subject to local taxes which are calculated during checkout ADDITIONAL ACCESS OPTIONS: * Log in

* Learn about institutional subscriptions * Read our FAQs * Contact customer support REFERENCES ORIGINAL RESEARCH PAPER * Wang, A. et al. The stoichiometric production of IL-2 and IFN-γ mRNA

defines memory T cells that can self-renew after adoptive transfer in humans. _Sci. Transl Med._ 4, 149ra120 (2012) PubMed  PubMed Central  Google Scholar  FURTHER READING * Gattinoni, L.,

Klebanoff, C. A. & Restifo, N. P. Paths to stemness: building the ultimate antitumour T cell. _Nature Rev. Cancer_ 12, 671–684 (2012) Article  CAS  Google Scholar  Download references

AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Acting Chief Editor, Nature Reviews Immunology, Olive Leavy Authors * Olive Leavy View author publications You can also search for this author

inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Leavy, O. Isolation of TCM cells for tumour immunotherapy. _Nat Rev Cancer_ 12,

656–657 (2012). https://doi.org/10.1038/nrc3364 Download citation * Published: 24 September 2012 * Issue Date: October 2012 * DOI: https://doi.org/10.1038/nrc3364 SHARE THIS ARTICLE Anyone

you share the following link with will be able to read this content: Get shareable link Sorry, a shareable link is not currently available for this article. Copy to clipboard Provided by the

Springer Nature SharedIt content-sharing initiative