The xanthophyll cycle affects reversible interactions between psbs and light-harvesting complex ii to control non-photochemical quenching

ABSTRACT To maintain high photosynthetic rates, plants must adapt to their light environment on a timescale of seconds to minutes. Therefore, the light-harvesting antenna system of

photosystem II in thylakoid membranes, light-harvesting complex II (LHCII), has a feedback mechanism, which determines the proportion of absorbed energy dissipated as heat: non-photochemical

chlorophyll fluorescence quenching (NPQ). This is crucial to prevent photo-oxidative damage to photosystem II (PSII) and is controlled by the transmembrane pH differences (ΔpH). High ΔpH

activates NPQ by protonation of the protein PsbS and the enzymatic de-epoxidation of LHCII-bound violaxanthin to zeaxanthin. But the precise role of PsbS and its interactions with different

LHCII complexes remain uncertain. We have investigated PsbS–LHCII interactions in native thylakoid membranes using magnetic-bead-linked antibody pull-downs. The interaction of PsbS with the

antenna system is affected by both ΔpH and the level of zeaxanthin. In the presence of ΔpH alone, PsbS is found to be mainly associated with the trimeric LHCII protein polypeptides, Lhcb1,

Lhcb2 and Lhcb3. However, a combination of ΔpH and zeaxanthin increases the proportion of PsbS bound to the minor LHCII antenna complex proteins Lhcb4, Lhcb5 and Lhcb6. This pattern of

interaction is not influenced by the presence of PSII reactions centres. Similar to LHCII particles in the photosynthetic membrane, PsbS protein forms clusters in the NPQ state. NPQ recovery

in the dark requires uncoupling of PsbS. We suggest that PsbS acts as a ‘seeding’ centre for the LHCII antenna rearrangement that is involved in NPQ. Access through your institution Buy or

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COMPLEX II IS AN ANTENNA OF PHOTOSYSTEM I IN DARK-ADAPTED PLANTS Article 22 June 2020 THE PSBS PROTEIN AND LOW PH ARE NECESSARY AND SUFFICIENT TO INDUCE QUENCHING IN THE LIGHT-HARVESTING

COMPLEX OF PLANTS LHCII Article Open access 01 April 2021 THE MOLECULAR PH-RESPONSE MECHANISM OF THE PLANT LIGHT-STRESS SENSOR PSBS Article Open access 16 April 2021 REFERENCES *

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Biophys. Acta_ 1817, 2–12 (2012). Article  Google Scholar  Download references ACKNOWLEDGEMENTS A.V.R. acknowledges funding from the Biotechnology and Biological Sciences Research Council of

the UK and the Leverhulme Trust. The authors acknowledge Y. Tian for the help with plant growth, as well as M. Johnson and C. Duffy for critical reading of the manuscript. AUTHOR

INFORMATION AUTHORS AND AFFILIATIONS * School of Biological and Chemical Sciences, Queen Mary University of London, Mile End Road, London, E1 4NS, UK Joanna Sacharz, Vasco Giovagnetti, Petra

Ungerer, Giulia Mastroianni & Alexander V. Ruban Authors * Joanna Sacharz View author publications You can also search for this author inPubMed Google Scholar * Vasco Giovagnetti View

author publications You can also search for this author inPubMed Google Scholar * Petra Ungerer View author publications You can also search for this author inPubMed Google Scholar * Giulia

Mastroianni View author publications You can also search for this author inPubMed Google Scholar * Alexander V. Ruban View author publications You can also search for this author inPubMed 

Google Scholar CONTRIBUTIONS J.S. and A.V.R. designed the experiments. J.S. performed biochemistry, PAM fluorescence measurements, preparation for TEM and particle analysis. V.G. performed

PAM fluorescence measurements. P.U. assisted with the experiments. G.M. operated TEM and assisted with sample preparation. All authors discussed the results and commented on the manuscript.

J.S., V.G. and A.V.R. wrote the manuscript. CORRESPONDING AUTHOR Correspondence to Alexander V. Ruban. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial

interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY INFORMATION Supplementary Figures 1–5. (PDF 923 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE

Sacharz, J., Giovagnetti, V., Ungerer, P. _et al._ The xanthophyll cycle affects reversible interactions between PsbS and light-harvesting complex II to control non-photochemical quenching.

_Nature Plants_ 3, 16225 (2017). https://doi.org/10.1038/nplants.2016.225 Download citation * Received: 06 October 2016 * Accepted: 22 December 2016 * Published: 30 January 2017 * DOI:

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