Modulation of glycine-activated ion channel function by g-protein βγ subunits

ABSTRACT Glycine receptors (GlyRs), together with GABAA and nicotinic acetylcholine (ACh) receptors, form part of the ligand-activated ion channel superfamily and regulate the excitability

of the mammalian brain stem and spinal cord. Here we report that the ability of the neurotransmitter glycine to gate recombinant and native ionotropic GlyRs is modulated by the G protein βγ

dimer (Gβγ). We found that the amplitude of the glycine-activated Cl− current was enhanced after application of purified Gβγ or after activation of a G protein–coupled receptor.

Overexpression of three distinct G protein α subunits (Gα), as well as the Gβγ scavenger peptide ct-GRK2, significantly blunted the effect of G protein activation. Single-channel recordings

from isolated membrane patches showed that Gβγ increased the GlyR open probability (_nP_o). Our results indicate that this interaction of Gβγ with GlyRs regulates both motor and sensory

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Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS CONFORMATIONAL TRANSITIONS AND ALLOSTERIC MODULATION IN A HETEROMERIC GLYCINE RECEPTOR Article Open access 13

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tissues. _J. Biol. Chem._ 274, 21425–21429 (1999). Article  CAS  Google Scholar  Download references ACKNOWLEDGEMENTS We thank S.R. Ikeda and N.L. Harrison for the plasmids, S.R. Ikeda for

critically reading the manuscript, and L.J. Aguayo, A. Ghazanfari and J.T. Healey for technical assistance. This work was supported by Fondecyt, GIA-DIUC (L.G.A. and J.O.), and by the

National Institute on Alcohol Abuse and Alcoholism intramural program (R.W.P.). AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Physiology, Laboratory of Neurophysiology, Box

160-C, University of Concepción, Chile Gonzalo E Yevenes, Juan C Tapia, Jorge Parodi & Luis G Aguayo * Unit on Cellular Neuropharmacology, Laboratory of Molecular and Cellular

Neurobiology, National Institute on Alcohol Abuse and Alcoholism, National Institutes of Health, Park 5 Building., Room. 150, 12420 Parklawn Dr., Bethesda, 20892-8115, Maryland, USA Robert W

Peoples * Department of Molecular Biology, Laboratory of Molecular Genetics, Box 160-C, University of Concepción, Chile Ximena Soto & Juan Olate Authors * Gonzalo E Yevenes View author

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Tapia View author publications You can also search for this author inPubMed Google Scholar * Jorge Parodi View author publications You can also search for this author inPubMed Google Scholar

* Ximena Soto View author publications You can also search for this author inPubMed Google Scholar * Juan Olate View author publications You can also search for this author inPubMed Google

Scholar * Luis G Aguayo View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Luis G Aguayo. ETHICS DECLARATIONS

COMPETING INTERESTS The authors declare no competing financial interests. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Yevenes, G., Peoples, R.,

Tapia, J. _et al._ Modulation of glycine-activated ion channel function by G-protein βγ subunits. _Nat Neurosci_ 6, 819–824 (2003). https://doi.org/10.1038/nn1095 Download citation *

Received: 29 April 2003 * Accepted: 23 May 2003 * Published: 13 July 2003 * Issue Date: 01 August 2003 * DOI: https://doi.org/10.1038/nn1095 SHARE THIS ARTICLE Anyone you share the following

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