A comprehensive map of human protein complexes

You have full access to this article via your institution. Download PDF Drew, K. _et al_. _Mol. Syst. Biol._ 13, 932 (2017). To better understand how protein complexes carry out their

cellular function or contribute to disease, it is essential to have a comprehensive map of interacting proteins. Drew _et al_. combined three prior large-scale mapping efforts based on

affinity purification mass spectrometry (AP-MS) or cofractionation MS of human cells. The authors integrated more than 9,000 MS experiments and developed a pipeline based on machine learning

techniques to first generate protein interaction networks and then cluster them. Reanalysis of the data yielded more than 15,000 new protein interactions revealing new complexes not

identified in the original data. In particular, the researchers focused on known and new complexes connected to ciliopathies and experimentally validated several of these complexes. Hu.MAP

is available at http://proteincomplexes.org/. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE A comprehensive map of human protein complexes. _Nat

Methods_ 14, 768 (2017). https://doi.org/10.1038/nmeth.4378 Download citation * Published: 01 August 2017 * Issue Date: 01 August 2017 * DOI: https://doi.org/10.1038/nmeth.4378 SHARE THIS

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