Making memories with crispr–cas9


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You have full access to this article via your institution. Download PDF Perli, S.D. _et al_. _Science_ http://dx.doi.org/10.1126/science.aag0511 (2016). Tracking the magnitude or duration of


biological activity in mammalian cells is technically challenging. Perli _et al_. devised a strategy that uses the CRISPR–Cas9 system to store such 'analog' information, called


mammalian synthetic cellular recorders integrating biological events (mSCRIBE). In mSCRIBE, a DNA sequence necessary for Cas9 recognition is added downstream of a gene that encodes the small


guide RNA, generating a self-targeting guide RNA (stgRNA) sequence. Then the expression of Cas9 is linked to a biological process of interest. When this process occurs, the Cas9 targets the


stgRNA gene for cleavage and subsequent mutagenesis during DNA repair. These mutations serve as a recording of when the process occurred. The researchers show that mSCRIBE can be used to


record induced acute inflammation events over time. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Making memories with CRISPR–Cas9. _Nat Methods_ 13,


904 (2016). https://doi.org/10.1038/nmeth.4051 Download citation * Published: 31 October 2016 * Issue Date: November 2016 * DOI: https://doi.org/10.1038/nmeth.4051 SHARE THIS ARTICLE Anyone


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