Large-scale de novo dna synthesis: technologies and applications

ABSTRACT For over 60 years, the synthetic production of new DNA sequences has helped researchers understand and engineer biology. Here we summarize methods and caveats for the _de novo_

synthesis of DNA, with particular emphasis on recent technologies that allow for large-scale and low-cost production. In addition, we discuss emerging applications enabled by large-scale _de

novo_ DNA constructs, as well as the challenges and opportunities that lie ahead. Access through your institution Buy or subscribe This is a preview of subscription content, access via your

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* Learn about institutional subscriptions * Read our FAQs * Contact customer support SIMILAR CONTENT BEING VIEWED BY OTHERS DNA SYNTHESIS TECHNOLOGIES TO CLOSE THE GENE WRITING GAP Article

23 January 2023 ENZYME-ASSISTED HIGH THROUGHPUT SEQUENCING OF AN EXPANDED GENETIC ALPHABET AT SINGLE BASE RESOLUTION Article Open access 14 May 2024 THE CHEMISTRY OF NEXT-GENERATION

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CAS  PubMed  Google Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Chemistry and Biochemistry, University of California, Los Angeles, Los Angeles,

California, USA Sriram Kosuri * Wyss Institute for Biologically Inspired Engineering, Boston, Massachusetts, USA George M Church * Department of Genetics, Harvard Medical School, Boston,

Massachusetts, USA George M Church Authors * Sriram Kosuri View author publications You can also search for this author inPubMed Google Scholar * George M Church View author publications You

can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Sriram Kosuri. ETHICS DECLARATIONS COMPETING INTERESTS S.K. and G.M.C. own stock in and are on

the Scientific Advisory Board of Gen9, a company that sells synthetic genes. G.M.C. is on the Board of Directors of Sigma-Aldrich and the Scientific Advisory Board of Cambrian Genomics,

both companies that sell synthetic genes or oligos. RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Kosuri, S., Church, G. Large-scale _de novo_ DNA

synthesis: technologies and applications. _Nat Methods_ 11, 499–507 (2014). https://doi.org/10.1038/nmeth.2918 Download citation * Received: 10 December 2013 * Accepted: 10 March 2014 *

Published: 29 April 2014 * Issue Date: May 2014 * DOI: https://doi.org/10.1038/nmeth.2918 SHARE THIS ARTICLE Anyone you share the following link with will be able to read this content: Get

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