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ABSTRACT Sub–diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected
per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 104−106 photons per
localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum. Access through your
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BYPASSES THE 10-NM RESOLUTION BARRIER Article Open access 01 August 2022 MINSTED NANOSCOPY ENTERS THE ÅNGSTRÖM LOCALIZATION RANGE Article Open access 07 November 2022 REFERENCES * Hell, S.W.
_Science_ 316, 1153–1158 (2007). Article CAS Google Scholar * Huang, B., Babcock, H. & Zhuang, X. _Cell_ 143, 1047–1058 (2010). Article CAS Google Scholar * Klar, T.A. & Hell,
S.W. _Opt. Lett._ 24, 954–956 (1999). Article CAS Google Scholar * Gustafsson, M.G.L. _Proc. Natl. Acad. Sci. USA_ 102, 13081–13086 (2005). Article CAS Google Scholar * Rust, M.J.,
Bates, M. & Zhuang, X. _Nat. Methods_ 3, 793–795 (2006). Article CAS Google Scholar * Betzig, E. et al. _Science_ 313, 1642–1645 (2006). Article CAS Google Scholar * Hess, S.T.,
Girirajan, T.P.K. & Mason, M.D. _Biophys. J._ 91, 4258–4272 (2006). Article CAS Google Scholar * Fölling, J. et al. _Nat. Methods_ 5, 943–945 (2008). Article Google Scholar *
Heilemann, M., van de Linde, S., Mukherjee, A. & Sauer, M. _Angew. Chem. Int. Ed. Engl._ 48, 6903–6908 (2009). Article CAS Google Scholar * Dempsey, G.T., Vaughan, J.C., Chen, K.H.,
Bates, M. & Zhuang, X.W. _Nat. Methods_ 8, 1027–1036 (2011). Article CAS Google Scholar * Patterson, G., Davidson, M., Manley, S. & Lippincott-Schwartz, J. _Annu. Rev. Phys.
Chem._ 61, 345–367 (2010). Article CAS Google Scholar * Hoyer, P., Staudt, T., Engelhardt, J. & Hell, S.W. _Nano Lett._ 11, 245–250 (2011). Article CAS Google Scholar * Yildiz, A.
et al. _Science_ 300, 2061–2065 (2003). Article CAS Google Scholar * Pertsinidis, A., Zhang, Y. & Chu, S. _Nature_ 466, 647–651 (2010). Article CAS Google Scholar * Tiers, G.V.D.
& Wiese, J.A. Jr. US Patent 3,916,069 (1975). * Kundu, K. et al. _Angew. Chem. Int. Ed. Engl._ 48, 299–303 (2009). Article CAS Google Scholar * Vogelsang, J. et al. _ChemPhysChem_ 11,
2475–2490 (2010). Article CAS Google Scholar * Ries, J., Kaplan, C., Platonova, E., Eghlidi, H. & Ewers, H. _Nat. Methods_ 9, 582–584 (2012). Article CAS Google Scholar *
Engelhardt, J. et al. _Nano Lett._ 11, 209–213 (2011). Article CAS Google Scholar * Enderlein, J., Toprak, E. & Selvin, P.R. _Opt. Express_ 14, 8111–8120 (2006). Article CAS Google
Scholar * Huang, B., Jones, S.A., Brandenburg, B. & Zhuang, X. _Nat. Methods_ 5, 1047–1052 (2008). Article CAS Google Scholar * Huang, B., Wang, W., Bates, M. & Zhuang, X.
_Science_ 319, 810–813 (2008). Article CAS Google Scholar * Bates, M., Huang, B., Dempsey, G.T. & Zhuang, X. _Science_ 317, 1749–1753 (2007). Article CAS Google Scholar * Galston,
A.W. _Proc. Natl. Acad. Sci. USA_ 35, 10–17 (1949). Article CAS Google Scholar * Vogelsang, J. et al. _Angew. Chem. Int. Ed. Engl._ 47, 5465–5469 (2008). Article CAS Google Scholar *
Rasnik, I., McKinney, S.A. & Ha, T. _Nat. Methods_ 3, 891–893 (2006). Article CAS Google Scholar * Patil, P.V. & Ballou, D.P. _Anal. Biochem._ 286, 187–192 (2000). Article CAS
Google Scholar Download references ACKNOWLEDGEMENTS We thank T. Mitchison, M. Ericsson and W. Wang for help during this project. This work is supported in part by the US National Institutes
of Health and a Collaborative Innovation Award from the Howard Hughes Medical Institute (to X.Z.). J.C.V. is supported in part by a Burroughs-Wellcome Career Award at the Scientific
Interface. X.Z. is a Howard Hughes Medical Institute investigator. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Howard Hughes Medical Institute, Harvard University, Cambridge,
Massachusetts, USA Joshua C Vaughan, Shu Jia & Xiaowei Zhuang * Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA Joshua C Vaughan, Shu Jia
& Xiaowei Zhuang * Department of Physics, Harvard University, Cambridge, Massachusetts, USA Xiaowei Zhuang Authors * Joshua C Vaughan View author publications You can also search for
this author inPubMed Google Scholar * Shu Jia View author publications You can also search for this author inPubMed Google Scholar * Xiaowei Zhuang View author publications You can also
search for this author inPubMed Google Scholar CONTRIBUTIONS J.C.V. is primarily responsible for experimental design. J.C.V. and S.J. performed experiments and analysis. X.Z. supervised and
guided the project. J.C.V. and X.Z. wrote the manuscript. CORRESPONDING AUTHOR Correspondence to Xiaowei Zhuang. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing
financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–9, Supplementary Note and Supplementary Protocol (PDF 9669 kb) RIGHTS AND PERMISSIONS
Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Vaughan, J., Jia, S. & Zhuang, X. Ultrabright photoactivatable fluorophores created by reductive caging. _Nat Methods_ 9,
1181–1184 (2012). https://doi.org/10.1038/nmeth.2214 Download citation * Received: 16 June 2012 * Accepted: 27 September 2012 * Published: 28 October 2012 * Issue Date: December 2012 * DOI:
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