Ultrabright photoactivatable fluorophores created by reductive caging

ABSTRACT Sub–diffraction-limit imaging can be achieved by sequential localization of photoactivatable fluorophores, for which the image resolution depends on the number of photons detected

per localization. We report a strategy for fluorophore caging that creates photoactivatable probes with high photon yields. Upon photoactivation, these probes can provide 104−106 photons per

localization and allow imaging of fixed samples with resolutions of several nanometers. This strategy can be applied to many fluorophores across the visible spectrum. Access through your

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Google Scholar  Download references ACKNOWLEDGEMENTS We thank T. Mitchison, M. Ericsson and W. Wang for help during this project. This work is supported in part by the US National Institutes

of Health and a Collaborative Innovation Award from the Howard Hughes Medical Institute (to X.Z.). J.C.V. is supported in part by a Burroughs-Wellcome Career Award at the Scientific

Interface. X.Z. is a Howard Hughes Medical Institute investigator. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Howard Hughes Medical Institute, Harvard University, Cambridge,

Massachusetts, USA Joshua C Vaughan, Shu Jia & Xiaowei Zhuang * Department of Chemistry and Chemical Biology, Harvard University, Cambridge, Massachusetts, USA Joshua C Vaughan, Shu Jia 

& Xiaowei Zhuang * Department of Physics, Harvard University, Cambridge, Massachusetts, USA Xiaowei Zhuang Authors * Joshua C Vaughan View author publications You can also search for

this author inPubMed Google Scholar * Shu Jia View author publications You can also search for this author inPubMed Google Scholar * Xiaowei Zhuang View author publications You can also

search for this author inPubMed Google Scholar CONTRIBUTIONS J.C.V. is primarily responsible for experimental design. J.C.V. and S.J. performed experiments and analysis. X.Z. supervised and

guided the project. J.C.V. and X.Z. wrote the manuscript. CORRESPONDING AUTHOR Correspondence to Xiaowei Zhuang. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing

financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY TEXT AND FIGURES Supplementary Figures 1–9, Supplementary Note and Supplementary Protocol (PDF 9669 kb) RIGHTS AND PERMISSIONS

Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Vaughan, J., Jia, S. & Zhuang, X. Ultrabright photoactivatable fluorophores created by reductive caging. _Nat Methods_ 9,

1181–1184 (2012). https://doi.org/10.1038/nmeth.2214 Download citation * Received: 16 June 2012 * Accepted: 27 September 2012 * Published: 28 October 2012 * Issue Date: December 2012 * DOI:

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