Microsatellite alterations in serum dna of head and neck cancer patients

ABSTRACT Microsatellite DNA alterations are an integral part of neoplastic progression and are valuable as clonal markers for the detection of human cancers1–3. Moreover, recent evidence

suggests that senescent tumor cells may release DNA into the circulation, which is subsequently carried by and therefore enriched in the serum and plasma4,5. We tested 21 patients with

primary head and neck squamous cell carcinoma (HNSCC) by polymerase chain reaction (PCR)–based microsatellite analysis of DNA from lymphocytes and paired serum samples. Patients were scored

for alterations as defined by the presence of new alleles (shifts) or loss of heterozygosity (LOH) in serum at each of 12 markers and then compared with primary tumor DNA. Six out of 21

patients (29%) were found to have one or more microsatellite alterations in serum precisely matching those in the primary tumors. All six patients had advanced disease (stage III or IV);

five of these patients had nodal metastases, three later developed distant metastases, and four died of disease. Microsatellite analysis of serum represents a novel method for the detection

of circulating tumor cell DNA. If these results are confirmed in larger studies, microsatellite markers may be useful in assessing tumor burden in cancer patients. Access through your

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(Lippincott, Philadelphia, 1988)._ Download references AUTHOR INFORMATION Author notes * David Sidransky: Correspondence should be addressed to D.S. AUTHORS AND AFFILIATIONS * Department of

Otolaryngology — Head and Neck Surgery, Division of Head and Neck Cancer Research, Johns Hopkins University School of Medicine, 818 Ross Research Building, 720 Rutland Avenue, Baltimore,

Maryland, 21205-2196 Homaira Nawroz, Wayne Koch & David Sidransky * laboratory of Plant Biochemistry and Physiology, Pavilion des Isotopes, Faculty of Science, University of Geneva, 20

boulevard d'Yvoy, 1211, Geneva, 4, Switzerland Philippe Anker & Maurice Stroun Authors * Homaira Nawroz View author publications You can also search for this author inPubMed Google

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for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Nawroz, H., Koch, W., Anker, P. _et al._ Microsatellite

alterations in serum DNA of head and neck cancer patients. _Nat Med_ 2, 1035–1037 (1996). https://doi.org/10.1038/nm0996-1035 Download citation * Received: 15 May 1996 * Accepted: 24 July

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