Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato

ABSTRACT Compared with vaccine delivery by injection, oral vaccines offer the hope of more convenient immunization strategies and a more practical means of implementing universal vaccination

programs throughout the world. Oral vaccines act by stimulating the immune system at effector sites (lymphoid tissue) located in the gut. Genetic engineering has been used with variable

success to design living and non-living systems as a means to deliver antigens to these sites and to stimulate a desired immune response1–4. More recently, plant biotechnology techniques

have been used to create plants which contain a gene derived from a human pathogen; the resultant plant tissues will accumulate an antigenic protein encoded by the foreign DNA5–10. In

pre-clinical trials, we found that antigenic proteins produced in transgenic plants retained immunogenic properties when purified; if injected into mice the antigen caused production of

protein-specific antibodies6. Moreover, in some experiments, if the plant tissues were simply fed to mice, a mucosal immune response occurred7–10. The present study was conducted as a proof

of principle to determine if humans would also develop a serum and/or mucosal immune response to an antigen delivered in an uncooked foodstuff. Access through your institution Buy or

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PREVENTION OF SHIGA TOXIN 1-CAUSED COLON INJURY BY PLANT-DERIVED RECOMBINANT IGA Article Open access 26 October 2022 PLANT VIRUS PARTICLES WITH VARIOUS SHAPES AS POTENTIAL ADJUVANTS Article

Open access 25 June 2020 INTEGRATING PLANT MOLECULAR FARMING AND MATERIALS RESEARCH FOR NEXT-GENERATION VACCINES Article 06 December 2021 REFERENCES * Tacket, C.O. _et al_. Comparison of

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Scholar  Download references AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Center for Vaccine Development, Department of Medicine, University of Maryland School of Medicine, Baltimore,

Maryland, 21201 Carol O. Tacket, Genevieve Losonsky & Myron M. Levine * Boyce Thompson Institute for Plant Research, Inc., Tower Road, Ithaca, New York, 14853-1801 Hugh S. Mason & 

Charles J. Arntzen * Tulane University School of Medicine, Department of Microbiology and Immunology, 1430 Tulane Avenue, New Orleans, Louisiana, 70112 John D. Clements Authors * Carol O.

Tacket View author publications You can also search for this author inPubMed Google Scholar * Hugh S. Mason View author publications You can also search for this author inPubMed Google

Scholar * Genevieve Losonsky View author publications You can also search for this author inPubMed Google Scholar * John D. Clements View author publications You can also search for this

author inPubMed Google Scholar * Myron M. Levine View author publications You can also search for this author inPubMed Google Scholar * Charles J. Arntzen View author publications You can

also search for this author inPubMed Google Scholar RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Tacket, C., Mason, H., Losonsky, G. _et al._

Immunogenicity in humans of a recombinant bacterial antigen delivered in a transgenic potato. _Nat Med_ 4, 607–609 (1998). https://doi.org/10.1038/nm0598-607 Download citation * Received: 05

March 1998 * Accepted: 05 April 1998 * Issue Date: 01 May 1998 * DOI: https://doi.org/10.1038/nm0598-607 SHARE THIS ARTICLE Anyone you share the following link with will be able to read

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