In vivo migration and function of transferred hiv-1-specific cytotoxic t cells

ABSTRACT THE PERSISTENCE OF HIV REPLICATION IN INFECTED INDIVIDUALS MAY REFLECT AN INADEQUATE HOST HIV-SPECIFIC CD8 + CYTOTOXIC T LYMPHOCYTE (CTL) RESPONSE. THE FUNCTIONAL ACTIVITY OF

HIV-SPECIFIC CTLS AND THE ABILITY OF THESE EFFECTOR CELLS TO MIGRATE _IN VIVO_ TO SITES OF INFECTION WAS DIRECTLY ASSESSED BY EXPANDING AUTOLOGOUS HIV-1 GAG-SPECIFIC CD8 + CTL CLONES _IN

VITRO_ AND ADOPTIVELY TRANSFERRING THESE CTLS TO HIV-INFECTED INDIVIDUALS. THE TRANSFERRED CTLS RETAINED LYTIC FUNCTION _IN VIVO_ , ACCUMULATED ADJACENT TO HIV-INFECTED CELLS IN LYMPH NODES

AND TRANSIENTLY REDUCED THE LEVELS OF CIRCULATING PRODUCTIVELY INFECTED CD4 + T CELLS. THESE RESULTS PROVIDE DIRECT EVIDENCE THAT HIV-SPECIFIC CTLS TARGET SITES OF HIV REPLICATION AND

MEDIATE ANTIVIRAL ACTIVITY, AND INDICATE THAT THE DEVELOPMENT OF IMMUNOTHERAPEUTIC APPROACHES TO SUSTAIN A STRONG CTL RESPONSE TO HIV MAY BE A USEFUL ADJUNCT TO TREATMENT OF HIV INFECTION.

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support SIMILAR CONTENT BEING VIEWED BY OTHERS IMMUNE MOBILISING T CELL RECEPTORS REDIRECT POLYCLONAL CD8+ T CELLS IN CHRONIC HIV INFECTION TO FORM IMMUNOLOGICAL SYNAPSES Article Open access

01 November 2022 CD8 LYMPHOCYTES MITIGATE HIV-1 PERSISTENCE IN LYMPH NODE FOLLICULAR HELPER T CELLS DURING HYPERACUTE-TREATED INFECTION Article Open access 12 July 2022 DYNAMIC CD4+ T CELL

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(1994). Download references ACKNOWLEDGEMENTS _We thank D. Spach (AIDS Clinical Trial Unit, University of Washington) for patient referral; K. Diem, M. Elliot, S. Friborg (Targeted Genetics),

C. Hoffer, D. Kelly, C. B. Joiner, J. Joyce, V. Mosiman, D. Muthui, P.M. O'Hearn, E.L. Peterson, L. Wilson (Targeted Genetics) and B. Wood for technical assistance; B. Gilman and E.

Gilman for their gift to the Program in Immunology; and Targeted Genetics for obtaining the IND for these studies, for providing the LN retroviral vector and for doing quality control

testing. This study was supported by funds from the National Institutes of Health (AI36613 and AI41535 to S.B., P30 HD28834 and AI-27757 to D.L., AI30731 to S.R., P.D.G, and L.C.) and from

the Pediatric AIDS Foundation, 77296-19—PF to D.L._ AUTHOR INFORMATION Author notes * Bruce K. Patterson, Daniel Jiyamapa and Philip D. Greenberg: Department of Obstetrics and Gynecology,

Northwestern University Medical School, Chicago, Illinois 94404, USA S.J.B. and D.A.L. contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of Laboratory Medicine, Box

356527, University of Washington, Seattle, 98101, Washington, USA Scott J. Brodie & Lawrence Corey * Department of Pediatrics, Box 356527, University of Washington, Seattle, 98101,

Washington, USA Deborah A. Lewinsohn * Department of Urology, Box 356527, University of Washington, Seattle, 98101, Washington, USA John Krieger * Department of Medicine, Box 356527,

University of Washington, Seattle, 98101, Washington, USA Lawrence Corey, Philip D. Greenberg & Stanley R. Riddell * Department of Immunology, Box 356527, University of Washington,

Seattle, 98101, Washington, USA Philip D. Greenberg * Fred Hutchinson Cancer Research Center, 1100 Fairview Ave. N., D3-100, Seattle, 98109, Washington , USA Deborah A. Lewinsohn, Lawrence

Corey, Philip D. Greenberg & Stanley R. Riddell Authors * Scott J. Brodie View author publications You can also search for this author inPubMed Google Scholar * Deborah A. Lewinsohn View

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ABOUT THIS ARTICLE CITE THIS ARTICLE Brodie, S., Lewinsohn, D., Patterson, B. _et al._ _In vivo_ migration and function of transferred HIV-1-specific cytotoxic T cells. _Nat Med_ 5, 34–41

(1999). https://doi.org/10.1038/4716 Download citation * Received: 29 July 1998 * Accepted: 05 November 1998 * Issue Date: January 1999 * DOI: https://doi.org/10.1038/4716 SHARE THIS ARTICLE

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