Epigenetic maintenance of the vernalized state in arabidopsis thaliana requires like heterochromatin protein 1

ABSTRACT Vernalization is the process by which sensing a prolonged exposure to winter cold leads to competence to flower in the spring. In winter annual _Arabidopsis thaliana_ accessions,

flowering is suppressed in the fall by expression of the potent floral repressor FLOWERING LOCUS C (FLC)1. Vernalization promotes flowering via epigenetic repression of _FLC_2. Repression is

accompanied by a series of histone modifications of _FLC_ chromatin that include dimethylation of histone H3 at Lys9 (H3K9) and Lys27 (H3K27)3,4. Here, we report that _A. thaliana_ LIKE

HETEROCHROMATIN PROTEIN 1 (LHP1) is necessary to maintain the epigenetically repressed state of _FLC_ upon return to warm conditions typical of spring. LHP1 is enriched at _FLC_ chromatin

after prolonged exposure to cold, and LHP1 activity is needed to maintain the increased levels of H3K9 dimethylation at _FLC_ chromatin that are characteristic of the vernalized state.

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support SIMILAR CONTENT BEING VIEWED BY OTHERS COLD-INDUCED _ARABIDOPSIS_ FRIGIDA NUCLEAR CONDENSATES FOR _FLC_ REPRESSION Article Open access 03 November 2021 DURATION OF COLD EXPOSURE

DEFINES THE RATE OF REACTIVATION OF A PERENNIAL _FLC_ ORTHOLOGUE VIA H3K27ME3 ACCUMULATION Article Open access 29 September 2020 HEAT SHOCK TRANSCRIPTION FACTOR B2B ACTS AS A TRANSCRIPTIONAL

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Download references ACKNOWLEDGEMENTS We are grateful to S. Woody, R. Schmitz and R. Rodman for their comments on the manuscript. Research in the laboratory of R.M.A. was supported by the

College of Agricultural and Life Sciences of the University of Wisconsin and by grants from the US Department of Agriculture National Research Initiative Competitive Grants Program and the

National Science Foundation. Research in the laboratory of S.E.J. was supported by US National Institute of Health grant GM060398. AUTHOR INFORMATION Author notes * Yuehui He Present

address: Department of Biological Sciences, Faculty of Science, National University of Singapore, Science Drive 4, Singapore, 117543 * Kenji Nakahigashi & Koji Goto Present address:

Institute for Advanced Biosciences, Yamagata, 997-0017, Japan * Sibum Sung and Yuehui He: These authors contributed equally to this work. AUTHORS AND AFFILIATIONS * Department of

Biochemistry, University of Wisconsin at Madison, Madison, Wisconsin, USA Sibum Sung, Yuehui He, Tifani W Eshoo, Yosuke Tamada & Richard M Amasino * Department of Molecular, Howard

Hughes Medical Institute, Cell, and Developmental Biology, and Molecular Biology Institute, University of California, Los Angeles, 90095, California, USA Lianna Johnson & Steve E

Jacobsen * Research Institute for Biological Sciences, Okayama, 716-1241, Japan Kenji Nakahigashi & Koji Goto * Department of Biological Sciences, Faculty of Science, National University

of Singapore, Science Drive 4, Singapore, 117543 Kenji Nakahigashi & Koji Goto * Institute for Advanced Biosciences, Yamagata, 997-0017, Japan Yuehui He Authors * Sibum Sung View author

publications You can also search for this author inPubMed Google Scholar * Yuehui He View author publications You can also search for this author inPubMed Google Scholar * Tifani W Eshoo

View author publications You can also search for this author inPubMed Google Scholar * Yosuke Tamada View author publications You can also search for this author inPubMed Google Scholar *

Lianna Johnson View author publications You can also search for this author inPubMed Google Scholar * Kenji Nakahigashi View author publications You can also search for this author inPubMed 

Google Scholar * Koji Goto View author publications You can also search for this author inPubMed Google Scholar * Steve E Jacobsen View author publications You can also search for this

author inPubMed Google Scholar * Richard M Amasino View author publications You can also search for this author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Richard M

Amasino. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. SUPPLEMENTARY INFORMATION SUPPLEMENTARY FIG. 1 LHP1 localization. (PDF 257 kb)

SUPPLEMENTARY TABLE 1 Primers used for ChIP and RT-PCR. (PDF 38 kb) RIGHTS AND PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Sung, S., He, Y., Eshoo, T. _et al._

Epigenetic maintenance of the vernalized state in _Arabidopsis thaliana_ requires LIKE HETEROCHROMATIN PROTEIN 1. _Nat Genet_ 38, 706–710 (2006). https://doi.org/10.1038/ng1795 Download

citation * Received: 22 November 2005 * Accepted: 05 April 2006 * Published: 07 May 2006 * Issue Date: 01 June 2006 * DOI: https://doi.org/10.1038/ng1795 SHARE THIS ARTICLE Anyone you share

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