Birc1e is the gene within the lgn1 locus associated with resistance to legionella pneumophila

ABSTRACT In inbred mouse strains, permissiveness to intracellular replication of _Legionella pneumophila_ is controlled by a single locus (_Lgn1_), which maps to a region within distal

Chromosome 13 that contains multiple copies of the gene baculoviral IAP repeat–containing 1 (_Birc1_, also called _Naip_; refs. 1–3). Genomic BAC clones from the critical interval were

transferred into transgenic mice to functionally complement the _Lgn1_-associated susceptibility of A/J mice to _L. pneumophila_. Here we report that two independent BAC clones that rescue

susceptibility have an overlapping region of 56 kb in which the entire _Lgn1_ transcript must lie. The only known full-length transcript coded in this region is _Birc1e_ (also called

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FOR VIRULENCE GENE INTERROGATION IN _LEGIONELLA PNEUMOPHILA_ Article Open access 04 February 2021 ACCESSION CODES ACCESSIONS GENBANK/EMBL/DDBJ * AF131205 * AF242431 * NM_010870 REFERENCES *

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  Google Scholar  Download references ACKNOWLEDGEMENTS The authors thank A. MacKenzie and D. Malo for their help and support, N. Gendron and J. Penney for expert technical assistance,

members of the McGill Mouse Genetics Group for use of the BAC transgenic core and L. Simard and C.J. DiDonato for BAC clone 227n6. This work was supported by grants from the Network of

Centers of Excellence (Canadian Genetic Diseases Network) to P.G. P.G. is supported by a Distinguished Scientist award and E.D. by a studentship from the Canadian Institutes of Health

Research. The BAC transgenic core was supported by a grant from Innovation Quebec. AUTHOR INFORMATION AUTHORS AND AFFILIATIONS * Department of Biochemistry, McGill University, 3655 Sir

William Osler Promenade, Montreal, H3G-1Y6, Quebec, Canada Eduardo Diez, Susan Gauthier, Michel Tremblay & Philippe Gros * Department of Biochemistry, Microbiology and Immunology,

University of Ottawa, Ottawa, Canada Seung-Hwan Lee & Silvia Vidal * Department of Pediatrics, University of Ottawa, Ottawa, Canada Zahra Yaraghi * McGill Cancer Center, McGill

University, Montreal, Canada Michel Tremblay & Philippe Gros Authors * Eduardo Diez View author publications You can also search for this author inPubMed Google Scholar * Seung-Hwan Lee

View author publications You can also search for this author inPubMed Google Scholar * Susan Gauthier View author publications You can also search for this author inPubMed Google Scholar *

Zahra Yaraghi View author publications You can also search for this author inPubMed Google Scholar * Michel Tremblay View author publications You can also search for this author inPubMed 

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author inPubMed Google Scholar CORRESPONDING AUTHOR Correspondence to Philippe Gros. ETHICS DECLARATIONS COMPETING INTERESTS The authors declare no competing financial interests. RIGHTS AND

PERMISSIONS Reprints and permissions ABOUT THIS ARTICLE CITE THIS ARTICLE Diez, E., Lee, SH., Gauthier, S. _et al._ _Birc1e_ is the gene within the _Lgn1_ locus associated with resistance to

_Legionella pneumophila_. _Nat Genet_ 33, 55–60 (2003). https://doi.org/10.1038/ng1065 Download citation * Received: 19 September 2002 * Accepted: 06 November 2002 * Published: 16 December

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